High-Density Lipoprotein Cholesterol (HDL-C) BioAssay™ Kit (Double Reagents)

Sensitivity: |The absorbance value △A is between 0.087~0.153 when testing 1.3mmol/L samples.||Range: |0.65-3.8mmol/L, r2>0.995||Accuracy: |Relative deviation ≤10%.||Precision: |Intra-Assay CV: <3%|Inter-Assay CV: <5%||Kit Components (see Protocol for final kit quantities):|Reagent 1, 75 mLx1 vial:|Good’s buffer, 50mmol/L|Toos, 1mmol/L|MgCl2·6H2O, 15mmol/L|Cholesterol oxidase, ≥3 KU|Peroxidase, ≥5 KU||Reagent 2, 25 mLx1 vial:|Good’s buffer, 50mmol/L|4-amino-antipyrine, 0.2mmol/L|MgCl2·6H2O, 15mmol/L|Cholesterol esterase, ≥3 KU|Surfactant, 0.1%||Standard, 0.1 mLx1 vial:|Cholesterol, 1.8mmol/L||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.