Goat anti-Rabbit IgG (H&L), DyLight® 594 conjugated
Référence AS111815
Conditionnement : 1mg
Marque : Agrisera
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AS11 1815 | Clonality: Polyclonal | Host: Goat | Reactivity: Rabbit IgG (H&L)
- Product Info
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Immunogen: Purified Rabbit IgG, whole molecule
Host: Goat Clonality: Polyclonal Purity: Immunogen affinity purified goat IgG. Format: Lyophilized Quantity: 1 mg Reconstitution: For reconstitution add 1,1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily Storage: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1,1 ml of sterile water add 1,1 ml of glycerol. Such solution will not freeze in -20°C, If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. Tested applications: Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunofluorescence (IF) Recommended dilution: 1 : 50- 1 : 5 000 (ICC), 1 : 20- 1 : 2000 (IHC), 1 : 3000 (IF) - Reactivity
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Confirmed reactivity: Rabbit IgG heavy and light chains (H&L) Predicted reactivity: Rabbit IgG Heavy and Light chains (H&L) Not reactive in: No confirmed exceptions from predicted reactivity are currently known - Additional Information
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Additional information: Conjugate is present in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) sodium azide is added as preservative.
DyLight® 594 (Ex = 593 nm; Em = 618 nm)
Additional information (application): Based in immunoelectrophoresis, this antibody reacts with heavy chains on rabbit IgG and light chains on all rabbit immunoglobulins.
No reactivity is observed to non-immunoglobulin rabbit serum proteins based in immunoelectrophoresis.
Purity of this antibody is 95% based on SDS-PAGE.
- Background
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Background: Goat anti-rabbit IgG, DyLight® 594 Conjugate is a secondary antibody conjugated to DyLight® 594, which binds to all rabbit IgGs in immunological assays.
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
- Product Citations
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Selected references: Chong et al. (2015). Active fungal GH115 α-glucuronidase produced in Arabidopsis thaliana affects only the UX1-reactive glucuronate decorations on native glucuronoxylans. BMC Biotechnol. 2015 Jun 18;15:56. doi: 10.1186/s12896-015-0154-8.
- Protocols
- Antibody protocols
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