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> Goat anti-FOXL2 / BPES

Goat anti-FOXL2 / BPES

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Référence EB06022

Conditionnement : 100ug

Marque : Everest Biotech

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Code Name Applications Availability Size Tested species Grade
EB06022 Goat Anti-FOXL2 / BPES Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Mouse
EB06022 1
EB06022 (2µg/ml) staining of Human (A) and (1µg/ml) Mouse (B) Ovary lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06022 2
EB06022 (5µg/ml) staining of paraffin embedded Human Ovary. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06022 3
EB06022 Negative Control showing staining of paraffin embedded Human Ovary, with no primary antibody.

Target Protein

Principal Names: FOXL2, BPES, forkhead box L2, PFRK, BPES1, PINTO, forkhead transcription factor FOXL2, blepharophimosis, epicanthus inversus and ptosis, Blepharophimosis, epicanthus inversus, and ptosis 1, POF3
Official Symbol: FOXL2
Accession Number(s): NP_075555.1
Human GeneID(s): 668
Non-Human GeneID(s): 26927 (mouse)

Immunogen

Peptide with sequence C-DSKTGALHSRLDL, from the C Terminus of the protein sequence according to NP_075555.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx. 50kDa band observed in Human and Mouse Ovary lysates (calculated MW of 38.8kDa according to Human NP_075555.1 and Mouse NP_036150.1). This molecular weight is observed by other Sources. Recommended concentration 1-3ug/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Ovary. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Cow

Specific Reference

This antibody (previous batch) has been successfully used in IF on Mouse:
Padua MB, Fox SC, Jiang T, Morse DA, Tevosian SG.
Simultaneous Gene Deletion of Gata4 and Gata6 Leads to Early Disruption of Follicular Development and Germ Cell Loss in the Murine Ovary.
Biol Reprod. 2014 Jun 4.
PMID: 24899573

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