Glycogen Colorimetric Assay Kit (Liver/Muscle Samples)

Référence E-BC-K073-S-50

Conditionnement : 50Assays

Marque : Elabscience

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Detection Principle Under the presence of concentrated sulfuric acid, glycogen can be dehydrated to furfural derivatives. Furfural derivatives can form blue compound with anthracenone. The concentration of the compound can be measured by colorimetric quantification at 620 nm with glucose standard buffer of same treatment. Glycogen is quite stable in concentrated alkali solution. Heating the tissue sample in concentrated alkali solution before color development will remove other components and keep the glycogen.
Sample Type Animal liver,Muscle
Detection Method Colorimetric method
Detection Instrument Spectrophotometer (620 nm)
Research Area Glycolysis And Lipid Metabolism
Other Reagents Required Normal saline (0.9% NaCl), Concentrated sulfuric acid
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 1.80 mg/g liver tiss
Detection Range 1.80-180 mg/g liver
Precision inter-assay CV: 7.3% | intra-assay CV: 3.7%
Sample Volume 100 μL(tissue homogenate)
Assay Time 50 min
Other Clones

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Other Formats

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Biotin

Chromogenic Biotin

Cyanine 3

Cyanine 5

Cyanine 5.5

Cyanine 7

Elab Fluor® Violet 450

Elab Fluor®430

Elab Fluor®488

Elab Fluor®568

Elab Fluor®594

Elab Fluor®647

Elab Fluor®680

Elab Fluor®700

Elab Fluor®750

Elab Fluor®Red 780

Elab Fluor®Violet 450

FITC

HRP

NHS-Biotin

NHS-LC-LC-Biotin

Sulfo-NHS-Biotin

Sulfo-NHS-LC-LC-Biotin

Unconjugated

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detection instrument

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