Glycerol-3-phosphate (G3P) Fluorometric Assay Kit
Référence E-BC-F081-96T
Conditionnement : 96T
Marque : Elabscience
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
| Detection Principle | Glycerol 3-phosphate (G3P), also known as 3-glycerophosphoic acid and glycerol α-phosphate, is mainly derived from glycolysis. G3P is an important intermediate in the glycerol-3-phosphate shuttle mechanism and studies have shown that G3P is involved in electron transport, and its metabolic level is related to lipid metabolism, insulin resistance and some cancers. The principle of this kit is that G3P is decomposed by enzymes, and its products generate fluorescent substances with probes under the action of other enzymes. The higher the fluorescence value, the higher the G3P content in the sample. |
| Synonyms | G3P |
| Sample Type | Tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=535 nm/587 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl), Double distilled water |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.19 μmol/L |
| Detection Range | 0.19-100 μmol/L |
| Precision | inter-assay CV: 4.6-10.0% | intra-assay CV: 0.7-2.8% |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 10% Mouse heart tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 1 × 10^6 293T cells | 1 |
| 1 × 10^6 HL-60 cells | 1 |
| 1 × 10^6 Hela cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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