Free Cholesterol (FC) Assay Kit is an assay kit for the detection and quantification of Free Cholesterol (FC) concentrations in serum, plasma and tissue homogenates using a microplate reader. Free cholesterol produces 4-cholestenone and hydrogen peroxide under the oxidation of cholesterol oxidase. In the presence of 4-aminoamylpyridine and phenol, peroxidase catalyze hydrogen peroxide to form red quinone compounds of benzoquinone imine phenizone. The color depth of the generated quinones is directly proportional to the cholesterol content.
Research Articles on Free Cholesterol (FC)
| Target | Free Cholesterol (FC) |
| Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
| Validity | The validity for this kit is 12 months. |
| Test Range | 0.07 mmol/L - 24 mmol/L |
| Sensitivity | 0.07 mmol/L |
| Detection Method | Colorimetric |
| Assay Data | Quantitative |
| Sample Type | Serum, plasma and tissue homogenates. |
| Availability | Shipped within 5-10 working days. |
| Note | This product is for research use only. |