Application

• For quantitative determination of triglyceride and evaluation of drug effects on triglyceride metabolism.

Key Features

• Sensitive and accurate. Use as little as 10 µL samples. Linear detection range from 0.01 to 1.0 mmol/L (0.88 to 88.5 mg/dL) triglyceride.
• Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature, compatible for HTS assays.
• Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.

Method

• OD570nm

Samples

• Serum, plasma etc

Species

• All

Procedure

• 30 min

Size

• 200 tests

Detection Limit

• 0.01 mmol/L (0.88 mg/dL)

Shelf Life

• 12 months

More Details

How to extract triglycerides from tissue?

1. Prepare extraction buffer with 5 vol isopropanol, 2 vol water, 2 vol Triton X-100.

In a 1.5 mL Eppendorf tube, add 50 µL/mg tissue of the extraction buffer. Vortex for at least 30 sec.

3. Centrifuge 5 min at 14,000 rpm on a table centrifuge.

4. Remove supernatant, dilute 5× with dH₂O and perform triglyceride assay using the ETGA-100 Kit.

I want to quantify triglycerides in algae and yeasts. The kit’s datasheet explains to use Triton X-100 to solubilize cells and solid components. Will this also work for Algae and Yeast?

Since yeast and algae have cell walls, Triton X-100 may not be sufficient to solubilize these cells. Sigma Aldrich offers a couple reagents for lysis of yeast and plant cells which should work, although we have not tested if these reagents would interfere with the assay.

Alternatively,you could try using sonication/ mechanical homogenization to disrupt the cells.

Jiang, S. et al (2021). Associations of Circulating Irisin with FNDC5 Expression in Fat and Muscle in Type 1 and Type 2 Diabetic Mice. Biomolecules, 11(2), 322. Assay: tryglycerides in mouse plasma.

Lee, J. et al (2020). Pharmaceutical Efficacy of Gypenoside LXXV on Non-Alcoholic Steatohepatitis (NASH). Biomolecules, 10(10), E1426. Assay: tryglycerides in mouse liver tissue.

Andres-Hernando, A. et al (2020). Deletion of Fructokinase in the Liver or in the Intestine Reveals Differential Effects on Sugar-Induced Metabolic Dysfunction. Cell metabolism, 32(1), 117-127. Assay: tryglycerides in mouse liver tissue.

Jhun, J. et al (2021). GRIM19 Impedes Obesity by Regulating Inflammatory White Fat Browning and Promoting Th17/Treg Balance. Cells, 10(1), E162. Assay: tryglycerides in mouse liver tissue.

Moorhead, S. G.et al. Variation of body condition and plasma energy substrates with life stage, sex, and season in wild-sampled nurse sharks Ginglymostoma cirratum. Journal of fish biology. Assay: tryglycerides in nurse shark plasma.

Andres-Hernando, A. et al. Vasopressin mediates fructose-induced metabolic syndrome by activating the V1b receptor. JCI insight, 140848. Assay: tryglycerides in mouse liver tissue.

Chi, Y. et al (2020). PHTF2 Regulates Lipids Metabolism in Gastric Cancer. Aging, 12(8), 6600-6610. Assay: tryglycerides in human gastric cells.

Shin, M. K., Yang, S. M., & Han, I. S. (2020). Capsaicin suppresses liver fat accumulation in high-fat diet-induced NAFLD mice. Animal cells and systems, 24(4), 214-219. Assay: tryglycerides in mouse plasma.

Ghazali, R. et al (2020). High omega arachidonic acid/docosahexaenoic acid ratio induces mitochondrial dysfunction and altered lipid metabolism in human hepatoma cells. World journal of hepatology, 12(3), 84. Assay: tryglycerides in human hepatoma.

Shi, Y., & Leung, S. W. S. Long-term Nitric Oxide Synthase Inhibition Prevents 17β-estradiol-induced Suppression of Cyclooxygenase-Dependent Contractions and Enhancement of Endothelium-Dependent Hyperpolarization-Like Relaxation in Mesenteric Arteries of Ovariectomized Rats. European journal of pharmacology, 882, 173275. Assay: tryglycerides in rat plasma.

Han, Y. H., Kim, H. J., & Lee, M. O. RORα regulates hepatic lipolysis by inducing transcriptional expression of PNPLA3 in mice. Molecular and cellular endocrinology, 111122. Assay: tryglycerides in mouse hepatocytes.

Hirsova, P. et al (2020). Hepatocyte Apoptosis Is Tumor Promoting in Murine Nonalcoholic Steatohepatitis. Cell death & disease, 11(2), 80. Assay: tryglycerides in mouse liver homogenate.

Nguyen, H. T. L., Kasapis, S., & Mantri, N. (2021). Physicochemical Properties and Effects of Honeys on Key Biomarkers of Oxidative Stress and Cholesterol Homeostasis in HepG2 Cells. Nutrients, 13(1), 151. Assay: tryglycerides in human liver cells.

Bergmans, R. S. et al (2020). Comparison of Cricket Diet With Peanut-Based and Milk-Based Diets in the Recovery From Protein Malnutrition in Mice and the Impact on Growth, Metabolism and Immune Function. PloS one, 15(6), e0234559. Assay: tryglycerides in mouse serum.

Seo, B. et al (2020). Roseburia spp. Abundance Associates with Alcohol Consumption in Humans and Its Administration Ameliorates Alcoholic Fatty Liver in Mice. Cell host & microbe, 27(1), 25. Assay: tryglycerides in mouse liver.

Lambert, K. et al (2020). Biocompatible Modified Water as a Non-Pharmaceutical Approach to Prevent Metabolic Syndrome Features in Obesogenic Diet-Fed Mice. Food and chemical toxicology: an international journal published for the British Industrial Biological Research Association, 141, 111403. Assay: tryglycerides in mouse liver.

Ju, U. I. et al (2020). Neddylation of Sterol Regulatory Element-Binding Protein 1c Is a Potential Therapeutic Target for Nonalcoholic Fatty Liver Treatment. Cell death & disease, 11(4), 283. Assay: tryglycerides in human serum and liver.

Colijn, S. et al (2020). Cell-specific and Athero-Protective Roles for RIPK3 in a Murine Model of Atherosclerosis. Disease models & mechanisms, 13(1), dmm041962. Assay: tryglycerides in mouse plasma.

Azar, S. et al. “Reversal of diet-induced hepatic steatosis by peripheral CB1 receptor blockade in mice is p53/miRNA-22/SIRT1/PPARα dependent.” Molecular metabolism 42: 101087. Assay: tryglycerides in mouse liver.

Pereira, R. O. et al (2021). OPA1 Deletion in Brown Adipose Tissue Improves Thermoregulation and Systemic Metabolism via FGF21. bioRxiv, 2021-01. Assay: tryglycerides in mouse liver and serum.

Higashi, K., Mori, A., Sakamoto, K., Ishii, K., & Nakahara, T. (2019). Probucol Slows the Progression of Cataracts in Streptozotocin-Induced Hyperglycemic rats. Pharmacology, 103(3-4), 212-219. Assay: Triglyceride in rat plasma.

Zagury, Y., Chen, S., Edelman, R., Karnieli, E., & Livney, Y. D. (2019). beta-Lactoglobulin delivery system for enhancing EGCG biological efficacy in HFD obesity mice model. Journal of Functional Foods, 59, 362-370. Assay: Triglyceride in mouse liver.

Zhang, X., Wang, S., Hu, L., Wang, J., Liu, Y., & Shi, P. (2019). Gemfibrozil reduces lipid accumulation in SMMC-7721 cells via the involvement of PPARalpha and SREBP1. Experimental and therapeutic medicine, 17(2), 1282-1289. Assay: Triglyceride in human hepatoma SMMC-7721 cells.

Zuo, L., Ge, S., Ge, Y., Li, J., Zhu, B., Zhang, Z., & Li, S. (2019). The adipokine metrnl ameliorates chronic colitis in Il-10-/-mice by attenuating mesenteric adipose tissue lesions during spontaneous colitis. Journal of Crohn’s and Colitis. Assay: Triglyceride in mouse & human mesenteric adipose tissue.

Ding, Y., Cui, J., Wang, Q., Shen, S., Xu, T., Tang, H., & Wu, X. (2018). The Vitamin K Epoxide Reductase Vkorc1l1 Promotes Preadipocyte Differentiation in Mice. Obesity, 26(8), 1303-1311. Assay: Triglyceride in mouse adipocytes.

Feng, J., Li, L., Ou, Z., Li, Q., Gong, B., Zhao, Z., & Yang, X. (2018). IL-25 stimulates M2 macrophage polarization and thereby promotes mitochondrial respiratory capacity and lipolysis in adipose tissues against obesity. Cellular & molecular immunology, 15(5), 493. Assay: Triglyceride in mouse liver and serum.

Lambert, K., Hokayem, M., Thomas, C., Fabre, O., Cassan, C., Bourret, A., & Avignon, A. (2018). Combination of nutritional polyphenols supplementation with exercise training counteracts insulin resistance and improves endurance in high-fat diet-induced obese rats. Scientific reports, 8(1), 2885. Assay: Triglyceride in rat liver homogenate.

Buss, L. A., & Dachs, G. U. (2017). Voluntary exercise slows breast tumor establishment and reduces tumor hypoxia in ApoE-/- mice. Journal of Applied Physiology, 124(4), 938-949. Assay: Triglyceride in mouse serum.

Gallagher, A. J., Skubel, R. A., Pethybridge, H. R., & Hammerschlag, N. (2017). Energy metabolism in mobile, wild-sampled sharks inferred by plasma lipids. Conservation Physiology, 5(1). Assay: Triglyceride in shark plasma.

Kim, S. H., Kim, G., Han, D. H., Lee, M., Kim, I., Kim, B., & Bae, S. H. (2017). Ezetimibe ameliorates steatohepatitis via AMP activated protein kinase-TFEB-mediated activation of autophagy and NLRP3 inflammasome inhibition. Autophagy, 13(10), 1767-1781. Assay: Triglyceride in mouse Liver tissue.

Lee, H., Shim, E. H., Lee, M. S., & Myung, C. S. (2017). Traditional medicine, Sobokchukeo-Tang, modulates the inflammatory response in adipocytes and macrophages. Molecular medicine reports, 15(1), 117-124. Assay: Triglyceride in RAW264.7 and THP-1 cells cell lysate.

Sadowska, J., Gebczynski, A. K., & Konarzewski, M. (2017). Metabolic risk factors in mice divergently selected for BMR fed high fat and high carb diets. PloS one, 12(2), e0172892. Assay: Triglyceride in mouse serum.

Wang, R. Y., Abbott, R. D., Zieba, A., Borowsky, F. E., & Kaplan, D. L. (2017). Development of a three-dimensional adipose tissue model for studying embryonic exposures to obesogenic chemicals. Annals of biomedical engineering, 45(7), 1807-1818. Assay: Triglyceride in human embryonic derived stem cells.

Choi, Y., Abdelmegeed, M. A., & Song, B. J. (2016). Preventive effects of dietary walnuts on high-fat-induced hepatic fat accumulation, oxidative stress and apoptosis in mice. The Journal of nutritional biochemistry, 38, 70-80. Assay: Triglyceride in mouse liver tissue.

Hernandez-Vazquez, A. D. J., Garcia-Sanchez, J. A., Moreno-Arriola, E., Salvador-Adriano, A., Ortega-Cuellar, D., & Velazquez-Arellano, A. (2016). Thiamine deprivation produces a liver ATP deficit and metabolic and genomic effects in mice: findings are parallel to those of biotin deficiency and have implications for energy disorders. Lifestyle Genomics, 9(5-6), 287-299. Assay: Triglyceride in mouse liver tissue.

Liss, S. A., Lamer, J. T., Sass, G. G., & Suski, C. D. (2016). Physiological consequences of hybridization: early generation backcrossing decreases performance in invasive bigheaded carps. Journal of Freshwater Ecology, 31(4), 543-554. Assay: Triglyceride in big-headed and silver carp blood.

Marques, T. M., Patterson, E., Wall, R., O’Sullivan, O., Fitzgerald, G. F., Cotter, P. D., & Stanton, C. (2016). Influence of GABA and GABA-producing Lactobacillus brevis DPC 6108 on the development of diabetes in a streptozotocin rat model. Beneficial microbes, 7(3), 409-420. Assay: Triglyceride in mouse.

Patterson, T. G., Freeman, T., & Flook, J. A. (2016). Colorimetric determination of the total oil content of a plant tissue sample using alkaline saponification. U.S. Patent No. 9,395,377. Assay: Triglyceride in human kernel.

Zawieja, S. D., Wang, W., Chakraborty, S., Zawieja, D. C., & Muthuchamy, M. (2016). Macrophage alterations within the mesenteric lymphatic tissue are associated with impairment of lymphatic pump in metabolic syndrome. Microcirculation, 23(7), 558-570. Assay: Triglyceride in mouse serum.

Cylwik B et al (2012) Relationship between CDT and disease activity in rheumatoid arthritis. Z Rheumatol 71(3):220-3. Assay: Triglyceride in rat blood.

Bytautiene, E., et al. (2011). Prepregnancy obesity and sFlt1-induced preeclampsia in mice: developmental programming model of metabolic syndrome. Am J Obstet Gynecol 204(5):398 e1-8. Assay: Triglyceride in mouse serum.

Guo H, et al (2011). Anthocyanin inhibits high glucose-induced hepatic mtGPAT1 activation and prevents fatty acid synthesis through PKCzeta. J Lipid Res. 52(5):908-22. Assay: Triglyceride in mouse, human cellular fractions.

Orban T, et al (2011). Retinyl ester storage particles (retinosomes) from the retinal pigmented epithelium resemble lipid droplets in other tissues. J Biol Chem. 286(19):17248-58. Assay: Triglyceride in cow retinal pigment epithelium.

Uddin MJ, et al (2011). Detection of quantitative trait loci affecting serum cholesterol, LDL, HDL, and triglyceride in pigs. BMC Genet.12:62. Assay: Triglyceride in pig serum.

Hu, M. et al. (2010). Effect of prolonged starvation on body weight and blood-chemistry in two horseshoe crab species: Tachypleus tridentatus and Carcinoscorpius rotundicauda (Chelicerata: Xiphosura). J. Exp. Marine Biol. Ecology 395(1-2):112-119. Assay: Triglyceride in horseshoe crab plasma.

Kim HS et al (2010). Hepatic-specific disruption of SIRT6 in mice results in fatty liver formation due to enhanced glycolysis and triglyceride synthesis. Cell Metab. 12(3):224-36. Assay: Triglyceride in human HepG2 hepatocellular carcinoma cells.

Oh TW, et al (2010). Semipurified fractions from the submerged-culture broth of Agaricus blazei Murill reduce blood glucose levels in streptozotocin-induced diabetic rats. J Agric Food Chem. 58(7):4113-9. Assay: Triglyceride in rat plasma.

Tam J et al (2010). Peripheral CB1 cannabinoid receptor blockade improves cardiometabolic risk in mouse models of obesity. J Clin Invest. 120(8):2953-66. Assay: Triglyceride in mouse primary hepatocytes.

Tucci, S et al (2010). Medium-chain triglycerides impair lipid metabolism and induce hepatic steatosis in very long-chain acyl-CoA dehydrogenase (VLCAD)-deficient mice. Mol Genet Metab 101(1): 40-47 . Assay: Triglyceride in mouse Liver.

Lee, SM et al (2008).GCG-rich tea catechins are effective in lowering cholesterol and triglyceride concentrations in hyperlipidemic rats. Lipids 43(5): 419-429. . Assay: Triglyceride in rat plasma.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost