EnzyChrom™ Pyruvate Assay Kit
Application
- For quantitative determination of pyruvate and evaluation of drug effects on its metabolism.
Key Features
- Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 2 to 500 µM (17 µg/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 µM for fluorimetric assays.
- Simple and convenient. The procedure involves the addition of a single working reagent and incubation for 30 min at room temperature, compatible with HTS assays.
- Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.
Method
- OD570nm, or FL530/585nm
Samples
- Biological
Species
- All
Procedure
- 20 min
Size
- 100 tests
Detection Limit
- OD, FL: 2, 0.2 µM
Shelf Life
- 6 months
More Details
Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine, and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct, and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery. BioAssay Systems pyruvate assay uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to pyruvate concentration in the sample.I would like to use the EnzyChrom pyruvate assay kit (EYPR-100) to determine pyruvate levels in cell culture medium containing phenol red and fetal bovine serum. Does this interfere in any way with the measurement?
Phenol red adds a background to the OD measurement that will vary with the pH of the medium. Pyruvate in FBS will also add a background to the measurement. In order to correct for those interferences you should add a separate control with unused medium +FBS and subtract that from all sample values or, you could also prepare the standard in the medium + FBS. To play it safe. Alternatively you can use serum-free medium without phenol red.
Ahn, SJ et al (2020). The pta-acka pathway regulates lrgab-mediated pyruvate uptake in streptococcus mutans. Microorganisms, 8(6). Assay: Pyruvate in streptococcus mutans extracellular supernatant.
Hussein, NA et al (2021). Liraglutide treatment and acylcarnitine profiles in Egyptian obese insulin-resistant females. European Journal of Pharmacology, 891, 173668. Assay: Pyruvate in human plasma.
Lopez-Cano, C et al (2020). Effect of type 2 diabetes mellitus on the hypoxia-inducible factor 1-alpha expression. Is there a relationship with the clock genes? Journal of Clinical Medicine, 9(8). Assay: Pyruvate in human plasma.
Chao, CC et al (2019). Metabolic control of astrocyte pathogenic activity via cpla2-mavs. Cell, 179(7), 1483-1498.e22. Assay: Pyruvate in mouse mitochondria.
Ahn, SJ et al (2020). Regulation of cid and lrg expression by CodY in Streptococcus mutans. MicrobiologyOpen, 9(7), e1040. Assay: Pyruvate in streptococcus mutans extracellular supernatant.
Ahn, SJ et al (2020). Acetate and potassium modulate the stationary-phase activation of lrgab in streptococcus mutans. Frontiers in Microbiology, 11, 401. Assay: Pyruvate in streptococcus mutans extracellular supernatant.
Ahn, SJ et al (2020). Understanding lrgab regulation of streptococcus mutans metabolism. Frontiers in Microbiology, 11, 2119. Assay: Pyruvate in streptococcus mutans extracellular supernatant.
Choe, D., Lee, J. H., Yoo, M., Hwang, S., Sung, B. H., Cho, S., & Cho, B. K. (2019). Adaptive laboratory evolution of a genome-reduced Escherichia coli. Nature communications, 10(1), 935. Assay: Pyruvate in E.coli cell lysate.
Haouzi, P., McCann, M., Tubbs, N., Judenherc-Haouzi, A., Cheung, J., & Bouillaud, F. (2019). Antidotal Effects of the Phenothiazine Chromophore Methylene Blue Following Cyanide Intoxication. Toxicological Sciences,170(1):82-94 Assay: Pyruvate in rat serum.
Yanase, S., Yasuda, K., & Ishii, N. (2019). Monitoring Age-Related Changes in the Lactate/Pyruvate ratio Using a Colorimetric Assay in a C. elegans Model of Increased Life Span. In Pre-Clinical Models (pp. 123-132). humana Press, New York, NY. Assay: Pyruvate in C. elegans tissue.
Narain, N. R., & McCook, J. P. (2018). Methods for treatment of oncological disorders using an epimetabolic shifter (coenzyme Q10). U.S. Patent No. 9,896,731. Assay: Pyruvate in enzyme cells.
Schoenrogge, M., Kerndl, H., Zhang, X., Kumstel, S., Vollmar, B., & Zechner, D. (2018). alpha-cyano-4-hydroxycinnamate impairs pancreatic cancer cells by stimulating the p38 signaling pathway. Cellular signalling, 47, 101-108. Assay: Pyruvate in murine pancreatic cells.
Das, C., Wang, G., Sun, Q., & Ledden, B. (2017). Multiplexed and fully automated detection of metabolic biomarkers using microdialysis probe. Sensors and Actuators B: Chemical, 238, 633-640. Assay: Pyruvate in raw chemical.
Yanase, S., Suda, H., Yasuda, K., & Ishii, N. (2017). Impaired p53/CEP-1 is associated with lifespan extension through an age-related imbalance in the energy metabolism of C. elegans. Genes to Cells, 22(12), 1004-1010. Assay: Pyruvate in C. elegans tissue.
Zhao, C., Lin, Z., Dong, H., Zhang, Y., & Li, Y. (2017). Reexamination of the physiological role of PykA in Escherichia coli revealed that it negatively regulates the intracellular ATP levels under anaerobic conditions. Appl. Environ. Microbiol., 83(11), e00316-17. Assay: Pyruvate in E.coli cell lysate.
Yasid, N. A., Rolfe, M. D., Green, J., & Williamson, M. P. (2016). Homeostasis of metabolites in Escherichia coli on transition from anaerobic to aerobic conditions and the transient secretion of pyruvate. Royal Society open science, 3(8), 160187. Assay: Pyruvate in E.coli cell lysate.
Chen, X et al (2015). Terazosin activated Pgk1 and Hsp90 to promote stress resistance. Nat Chem Biol. 11(1):19-25. Assay: Pyruvate in fly cells.
Cui Z, Gao N, Wang Q, Ren Y, Wang K, Zhu T (2015). BcMctA, a putative monocarboxylate transporter, is required for pathogenicity in Botrytis cinerea. Curr Genet.61(4):545-53. Assay: Pyruvate in hyphae tissue.
Cajuday, LA (2014). Carbohydrate Uptake in Water Buffalo Cumulus-OocyteComplexes (COCs) Supplemented with RetinoicAcid During In Vitro Maturation. Philippine Journal of Science. 143(1): 43-48. Assay: Pyruvate in water buffalo ovary cells.
Verma MK et al (2014). Integrated analysis of chronic lipotoxicity on muscle metabolism and stress and its reversal by antioxidants. Springerplus 3: 251. Assay: Pyruvate in human cell lysate.
Wu J et al (2014). Pyruvate-associated acid resistance in bacteria. Appl Environ Microbiol. 80(14):4108-13. Assay: Pyruvate in E. coli cells.
Fong, JE et al (2013). Moderate excess of pyruvate augmentsosteoclastogenesis. Biol Open. 2(4):387-95. Assay: Pyruvate in mice serum.
Yasid, NAB (2013). Metabolite analysis of Escherichia coli in response to changes in oxygen levels. Department of Molecular Biology and Biotechnology,The University of Sheffield. Assay: Pyruvate in E. coli cells.
Darekar S, Georgiou K, Yurchenko M, Yenamandra SP, Chachami G, Simos G, Klein G, Kashuba E (2012). Epstein-Barr virus immortalization of human B-cells leads to stabilization of hypoxia-induced factor 1 alpha, congruent with the Warburg effect. PLoS One. 2012;7(7):e42072. Assay: Pyruvate in human cells.
Park MR, Baek SH, de Los Reyes BG, Yun SJ, Hasenstein KH (2012). Transcriptome profiling characterizes phosphate deficiency effects on carbohydrate metabolism in rice leaves. J Plant Physiol.169(2):193-205. Assay: Pyruvate in plant leaves.
Methods for treatment of oncological disorders using an epimetabolic shifter (coenzyme q10). US Patent Appl. 20110027247. Assay: Pyruvate in mouse cell.
Narain NR et al (2011). Methods For The Diagnosis Of Metabolic Disorders Using Epimetabolic Shiftersus Patent Applus 2011/0123986 A1. Assay: Pyruvate in human cell.
Sarangarajan, R. et al. (2011). Methods For Treatment Of A Sarcoma Using An Epimetabolic Shifter (Coenzyme Q10). US 2011/0064747 A1. Assay: Pyruvate in human cell.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost