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> Diatrizoic acid [117-96-4]

Diatrizoic acid [117-96-4]

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Diatrizoic acid (Diatrizoate) is an iodinated radiocontrast agent and has the potential for radiographic imaging of the airways. Diatrizoic acid induces mitochondrial turnover and oxidative stress, and activating apoptosis by dysregulating calcium.

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Diatrizoic acid Chemical Structure

Diatrizoic acid Chemical Structure

CAS No. : 117-96-4

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Description

Diatrizoic acid (Diatrizoate) is an iodinated radiocontrast agent and has the potential for radiographic imaging of the airways. Diatrizoic acid induces mitochondrial turnover and oxidative stress, and activating apoptosis by dysregulating calcium[1][2].

In Vitro

HK-2 cells are exposed to 0-30 mg I/mL Diatrizoic acid (Diatrizoate) or vehicle for 2-24 h. MTT and trypan blue exclusion indicate a decrease in mitochondrial and cell viability within 2 and 24 h, respectively[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Diatrizoic acid Related Antibodies
In Vivo

Diatrizoic acid is an iodinated radiocontrast agent. Histological examination of rat lung tissue shows no acute toxicity or inflammation after insufflation of Diatrizoic acid nanoparticle agglomerates. Diatrizoic acid nanoparticle agglomerates offers a promising radiocontrast agent for safe and effective lung visualization[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Essai clinique
Masse moléculaire

613.91

Formule

C11H9I3N2O4
CAS No.

117-96-4
Appearance

Solid

Color

White to off-white

SMILES

O=C(O)C1=C(I)C(NC(C)=O)=C(I)C(NC(C)=O)=C1I
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
Solvant et solubilité
In Vitro: 

DMSO : 100 mg/mL (162.89 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6289 mL 8.1445 mL 16.2890 mL
5 mM 0.3258 mL 1.6289 mL 3.2578 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

  • Calculateur de molarité

  • Calculateur de dilution

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (4.07 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (4.07 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation

Purity: 98.33%

SDS (393 KB)

COA (251 KB) HNMR (235 KB) LCMS (94 KB)

Instruction de manipulation (2659 KB)
Références

  • [1]. El-Gendy N, et al. Dry powdered aerosols of diatrizoic acid nanoparticle agglomerates as a lung contrast agent. Int J Pharm. 2010 May 31;391(1-2):305-12.  [Content Brief]

    [2]. Dakota B Ward, et al. Radiocontrast Agent Diatrizoic Acid Induces Mitophagy and Oxidative Stress via Calcium Dysregulation. Int J Mol Sci. 2019 Aug 21;20(17):4074.  [Content Brief]

Administration animale
[1]

Female Sprague-Dawley rats (200 to 250 grams) are housed in temperature and humidity controlled rooms with free access to food and water and maintained on a 12 h light/dark cycle. Rats are anesthetized by a 67.5 mg/kg ketamine, 3.5 mg/kg xylazine and 0.66 mg/kg acepromazine subcutaneous cocktail. While under anesthesia, rats are placed on a heating pad to maintain a body temperature of 37°C. Diatrizoic acid nanoparticle agglomerates (10 mg) is administered by intratracheal insufflation using a dry powder insufflator. At the end of the experiment, the rats are euthanized by isoflurane inhalation overdose followed by harvest of major organs[1].

MCE n'a pas confirmé de manière indépendante l'exactitude de ces méthodes. Ils sont pour référence seulement.
Références

  • [1]. El-Gendy N, et al. Dry powdered aerosols of diatrizoic acid nanoparticle agglomerates as a lung contrast agent. Int J Pharm. 2010 May 31;391(1-2):305-12.  [Content Brief]

    [2]. Dakota B Ward, et al. Radiocontrast Agent Diatrizoic Acid Induces Mitophagy and Oxidative Stress via Calcium Dysregulation. Int J Mol Sci. 2019 Aug 21;20(17):4074.  [Content Brief]

  • [1]. El-Gendy N, et al. Dry powdered aerosols of diatrizoic acid nanoparticle agglomerates as a lung contrast agent. Int J Pharm. 2010 May 31;391(1-2):305-12.

    [2]. Dakota B Ward, et al. Radiocontrast Agent Diatrizoic Acid Induces Mitophagy and Oxidative Stress via Calcium Dysregulation. Int J Mol Sci. 2019 Aug 21;20(17):4074.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.6289 mL 8.1445 mL 16.2890 mL 40.7226 mL
5 mM 0.3258 mL 1.6289 mL 3.2578 mL 8.1445 mL
10 mM 0.1629 mL 0.8145 mL 1.6289 mL 4.0723 mL
15 mM 0.1086 mL 0.5430 mL 1.0859 mL 2.7148 mL
20 mM 0.0814 mL 0.4072 mL 0.8145 mL 2.0361 mL
25 mM 0.0652 mL 0.3258 mL 0.6516 mL 1.6289 mL
30 mM 0.0543 mL 0.2715 mL 0.5430 mL 1.3574 mL
40 mM 0.0407 mL 0.2036 mL 0.4072 mL 1.0181 mL
50 mM 0.0326 mL 0.1629 mL 0.3258 mL 0.8145 mL
60 mM 0.0271 mL 0.1357 mL 0.2715 mL 0.6787 mL
80 mM 0.0204 mL 0.1018 mL 0.2036 mL 0.5090 mL
100 mM 0.0163 mL 0.0814 mL 0.1629 mL 0.4072 mL
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Diatrizoic acid Related Classifications

  • Apoptosis
  • Apoptosis
Help & FAQs

Keywords:

Diatrizoic acid117-96-4Diatrizoate Amidotrizoic acidApoptosisInhibitorinhibitorinhibit