Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> COX4I1 Monoclonal Antibody

COX4I1 Monoclonal Antibody

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Référence E-AB-22002-200

Conditionnement : 200uL

Marque : Elabscience

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Verified Samples Verified Samples in WB: Hela
Verified Samples in IHC: Rat kidney
Verified Samples in IF: Mouse kidney
Dilution WB 1:1000-3000,  IHC 1:50-300,  IF 1:100-1:300
Isotype IgG
Host Mouse
Reactivity Human,  Mouse,  Rat
Applications WB,  IHC-p,  IF
Clonality Monoclonal
Immunogen Recombinant Protein
Abbre COX IV
Synonyms AL024441,  COX 4,  COX IV,  COX IV 1,  COX IV-1,  COX41,  COX4B,  COX4I1,  COX4I2,  COX4L2,  COXIV,  Cox4,  Cox4a,  Cytochrome C Oxidas,  Cytochrome c oxidase polypeptide IV,  Cytochrome c oxidase subunit 4 isoform 1,  Cytochrome c oxidase subunit 4 isoform 1 mitochondrial,  mitochondrial
Swissprot
Calculated MW 20 kDa
Observed MW 15 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization Mitochondrion inner membrane.
Tissue Specificity Ubiquitous
Concentration 1 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol.
Purification Method Protein A purification
Research Areas Cancer,  Metabolism,  Signal Transduction,  Tags and Cell Markers
Clone No. 2D4
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it.
Cat.No. Product Name Sizes
E-AB-1001 Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1011 Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated) 500μL , 120μL , 60μL
E-AB-1015 Goat Anti-Mouse IgG(H+L)(FITC conjugated) 500μL , 120μL , 60μL
E-AB-1024 Goat Anti-Mouse IgG(H+L)(Biotin conjugated) 500μL , 120μL , 60μL
E-AB-1043 Streptavidin(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1059 Goat Anti-Mouse IgG(H+L)(Elab Fluor® 594 conjugated) 500μL , 120μL , 60μL
E-AB-1076 Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated) 500μL , 120μL , 60μL
E-AB-1196 HRP-Goat Anti-Mouse IgG(H+L) preadsorbed 500μL , 120μL , 1mL
E-IR-R217 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) 50mL , 18mL , 6mL , 3mL
E-IR-R220 Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) 10mL , 3mL
E-IR-R221 Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) 10mL , 3mL
E-IR-R304A Western Blot Detection Kit 50Assays
E-IR-R304B High Accuracy and Absorbability Western Blot Detection Kit 50Assays
Other Clones

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Unconjugated

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