Corticosterone ELISA Kit

Référence OKEH02545

Conditionnement : 96Wells

Marque : Aviva Systems Biology

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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH02545
Product Info
Predicted Species ReactivityAll Species
ApplicationELISA-Comp
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Corticosterone ELISA Kit (OKEH02545) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Corticosterone antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Corticosterone and incubated. The Corticosterone found in the sample or standards competes with the biotinylated Corticosterone for limited binding sites on the immobilized anti-Corticosterone antibody. Excess unbound biotinylated Corticosterone and sample or standard Corticosterone is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Corticosterone captured in the well and inversely proportional to the amount of Corticosterone which was contained in the sample or standard.
ELISA Kit Range7.8-500ng/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 97%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.2% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Corticosterone Microplate96 Wells (12 x 8 Well strips)
Corticosterone Lyophilized Standard2 x 500 ng
Sample Diluent1 x 20 mL
100X Corticosterone-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: CORTI
Target Name: Corticosterone
::Pubchem: C21H30O4
::Chemical formula: C21H30O4
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids
Sensitivity1.9 ng/mL
SpecificityNatural and recombinant General Corticosterone
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsCORTI, Corticosterone, CORTI
Protein NameCorticosterone
Description of TargetIn many species, including amphibians, reptiles, rodents and birds, corticosterone is a main glucocorticoid, involved in regulation of fuel, immune reactions, and stress responses. However, in humans, corticosterone is produced primarily in the zona fasciculata of the adrenal cortex. It has only weak glucocorticoid and mineralocorticoid potencies in humans and is important mainly as an intermediate in the steroidogenic pathway from pregnenolone to aldosterone. Corticosterone is converted to aldosterone by aldosterone synthase, found only in the mitochondria of glomerulosa cells. Glomerulosa cells are found in the Zona glomerulosa, which is the most superficial region of endocrine cells in the adrenal cortex. Corticosterone (CORT) is a 21-carbon steroid hormone of the corticosteroid type produced in the cortex of the adrenal glands.

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