CF®568 Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, 2 mg/mL

Référence 20101-1

Conditionnement : 50uL

Marque : Biotium

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Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed

Highly cross-adsorbed goat anti-mouse IgG (H L) secondary antibody labeled with our superior CF® Dyes.

Technology

CF® Dyes
Considerations for Immunofluorescence Staining
Secondary Antibody Product Listings

Tech Tips

Protocol: Fluorescent Western Blotting
Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host

Product Description

This is a highly cross-adsorbed goat anti-mouse IgG (H L) secondary antibody labeled with our bright and photostable CF® Dyes. To minimize cross-reactivity, the antibody has been adsorbed against bovine, horse, human, rabbit, and swine serum. This antibody is not cross-adsorbed against rat. For staining rat tissue, or co-staining with rat antibodies, we recommend using Goat Anti-Mouse IgG (H L), Highly Cross-Adsorbed (Min x Rat).

  • Highly-cross adsorbed for specific staining with minimal background
  • Available with 20 bright and photostable CF® Dyes
  • Biotin and HRP conjugates also available
  • Suitable for western, immunofluorescence, and immunohistology in FFPE tissues

Note: Conjugates of blue fluorescent dyes like CF®350 are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Goat Anti-Mouse IgG (H+L), Highly Cross-Adsorbed

Conjugation Ex/Em Size Catalog No. Dye Features
CF®350 347/448 nm 50 uL (100 ug) 20143-1 CF®350 Features
0.5 mL (1 mg) 20143
1 mg (lyophilized) 20143-1mg
CF®405M 408/452 nm 50 uL (100 ug) 20182-1 CF®405M Features
0.5 mL (1 mg) 20182
1 mg (lyophilized) 20182-1mg
CF®430 426/498 nm 50 uL (100 ug) 20459-50uL CF®430 Features
0.5 mL (1 mg) 20459-500uL
1 mg (lyophilized) 20459-1mg
CF®488A 490/515 nm 50 uL (100 ug) 20018-1 CF®488A Features
0.5 mL (1 mg) 20018
1 mg (lyophilized) 20018-1mg
CF®532 527/558 nm 50 uL (100 ug) 20468-50uL CF®532 Features
0.5 mL (1 mg) 20468-500uL
1 mg (lyophilized) 20468-1mg
CF®543 541/560 nm 50 uL (100 ug) 20299-1 CF®543 Features
0.5 mL (1 mg) 20299
1 mg (lyophilized) 20299-1mg
CF®555 555/565 nm 50 uL (100 ug) 20231-1 CF®555 Features
0.5 mL (1 mg) 20231
1 mg (lyophilized) 20231-1mg
CF®568 562/583 nm 50 uL (100 ug) 20101-1 CF®568 Features
0.5 mL (1 mg) 20101
1 mg (lyophilized) 20101-1mg
CF®583R 585/609 nm 50 uL (100 ug) 20904-50uL CF®583R Features
0.5 mL (1 mg) 20904-500uL
CF®594 593/614 nm 50 uL (100 ug) 20111-1 CF®594 Features
0.5 mL (1 mg) 20111
1 mg (lyophilized) 20111-1mg
CF®633 630/650 nm 50 uL (100 ug) 20121-1 CF®633 Features
0.5 mL (1 mg) 20121
1 mg (lyophilized) 20121-1mg
CF®640R 642/662 nm 50 uL (100 ug) 20175-1 CF®640R Features
0.5 mL (1 mg) 20175
1 mg (lyophilized) 20175-1mg
CF®647 650/665 nm 50 uL (100 ug) 20281-1 CF®647 Features
0.5 mL (1 mg) 20281
1 mg (lyophilized) 20281-1mg
CF®660C 667/685 nm 50 uL (100 ug) 20052-1 CF®660C Features
0.5 mL (1 mg) 20052
1 mg (lyophilized) 20052-1mg
CF®680R 680/701 nm 50 uL (100 ug) 20192-1 CF®680 Features
0.25 mL (500 ug) 20192
CF®680 681/698 nm 50 uL (100 ug) 20065-1 CF®680R Features
0.25 mL (500 ug) 20065
CF®740 742/767 nm 50 uL (100 ug) 20988-50uL CF®740 Features
250 uL (100 ug) 20988-250uL
CF®750 755/777 nm 50 uL (100 ug) 20463-50uL CF®750 Features
250 uL (100 ug) 20463-250uL
CF®770 770/797 nm 50 uL (100 ug) 20077-1 CF®770 Features
0.25 mL (500 ug) 20077
CF®790 784/806 nm 50 uL (100 ug) 20342-50uL CF®790 Features
Biotin N/A 50 uL (100 ug) 20184-1
0.5 mL (1 mg) 20184
1 mg (lyophilized) 20184-1mg
HRP N/A 100 uL (100 ug) 20401-100uL
1 mL (1 mg) 20401-1mL
1 mg (lyophilized) 20401-1mg

View our full selection of Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of CF® dye references.

 

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