Blocking Buffer Sampler Kit
Référence K505
Conditionnement : 1Kit
Marque : Rockland Immunochemicals
Specifications for Blocking Buffer Sampler Kit
Product Details
Blocking Buffer Sampler Kit - K505
Blocking Buffer Sampler Kit, Normal Goat Serum, NGS, BSA, Bovine Serum Albumin, Blocking Buffer for Fluorescent Western Blotting, ELISA Microwell Blocking Buffer with Stabilizer, TBS Fish Gel Solution
Sampler Pack
Target Details
Lyophilized normal goat serum was prepared from normal goat serum by a multi-step process which includes delipidation and selective precipitation. Assay by immunoelectrophoresis resulted in a multiple precipitin arcs against anti-Goat Serum. BSA has Purity (%): 100% by Agarose Zone Electrophoresis Protease: <0.005 units/mg by Enzymatic Assay. And no detectable IgG by Radial Immunodiffusion. Blocking Buffer (2X) for Fluorescent Western Blotting, ELISA Microwell blocking buffer with stabilizer, and Fish gel concentrate were aseptically filtered through a Millipore 0.22 micron filter into clean, pre-sterilized containers. The products were tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and found to be negative for bacteria.
Application Details
ELISA, IF, IHC, WB, Multiplex
Blocking Buffer Sampler Kit contains: Normal Goat Serum (NGS), Bovine Serum Albumin - Fraction V, Blocking Buffer (2X) for Fluorescent Western Blotting, ELISA Microwell Blocking Buffer with Stabilizer, 10X TBS Fish Gel Concentrate.
Formulation
See application note.
Shipping & Handling
Wet Ice
Do not freeze kit. Store kit at 4° C. Kit contains multiple components. See kit insert for complete instructions.
See kit insert for complete instructions.
Background
Set of different blocking buffers for all sorts of immunoassays.
Related Protocols
Adherent Cell Lysis Protocol
ELISA Protocol
Fluorescent Western Blotting Protocol
Heat-induced Antigen Retrieval Protocol
Histone Immunoblotting Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Immunohistochemistry (IHC) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Protease-induced Antigen Retrieval Protocol
Sandwich ELISA Protocol for Collagen
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Related Resources
Assay Substrate Selection Guide
Articles
View Article
Blocking Buffer Selection Guide
Articles
View Article
Streptavidin: Properties and Applications
Articles
View Article