Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> Blocking Buffer for Fluorescent Western Blotting

Blocking Buffer for Fluorescent Western Blotting

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Référence MB-070S

Conditionnement : 125mL

Marque : Rockland Immunochemicals

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Specifications for Blocking Buffer for Fluorescent Western Blotting

Product Details

Blocking Buffer for Fluorescent Western Blotting - MB-070
Multiplex Blocking Buffer, Fluorescent Blocking Buffer, Blocking Solution, Blocking Buffer Western Blot, IRDye Western Blot Blocking Buffer, Alexa Dye Blocking Buffer, DyLight Blocking Buffer

Target Details

Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerosal added as an antimicrobial agent.

Application Details

WB
Cellular Assay, ELISA, IF, IHC, IP, Microarray, Other  - View References
Fluorescence technology is widely used to detect proteins in both the visible and near-infrared ranges. This product allows for superior signal detection and lower background noise when fluorochrome conjugated antibodies are used to visualize proteins in Western blotting and other applications. Antibody conjugates prepared with IRDye® 800 and IRDye® 700DX, Cy2™, Cy3™, Cy3.5™, Cy5™ and Cy5.5™, DyLight™405, DyLight™ 549, DyLight™ 649, DyLight™ 680, and DyLight™ 800 and Alexa Fluor® 488, Alexa Fluor® 532, Alexa Fluor® 546, Alexa Fluor® 647 and Alexa Fluor® 680 have been validated on various platforms using this product with superior results compared to other commercially available products. In the infrared range, where little to no autofluorescence occurs, specific signal is sharply evident from any background giving the best possible signal-to-noise ratio. This allows for detection levels in the picogram range which rivals the sensitivity of chemiluminescence on film for Western blotting. Superior results are also seen when this product is used for simultaneous labeling (multiplex) in Western blots or microscopy using various fluorochrome combinations for multicolor imaging. Membranes blocked with this product can be dried and are very stable. Membranes that are stored protected from light can be re-washed and/or rescanned.

Formulation

1X
See application note.
Thimerosal is added as an antimicrobial agent.

Shipping & Handling

Wet Ice
Store Blocking Buffer at 4° C prior to opening. DO NOT FREEZE.
Expiration date is six (6) months from date of receipt.

Background

This blocking buffer is specifically designed for Western blotting using fluorochrome conjugated antibodies. Pure nitrocellulose membrane is recommended for maximum performance. Other membranes, such as PVDF or nitrocellulose embedded in a support can be used, but may generate elevated backgrounds. Protein should be transferred from gel to membrane using standard protocols. Blocking buffer can be used for membrane blocking and to dilute both primary and secondary antibodies. Blocking Buffer for Fluorescent Western Blotting is suitable for use with imaging systems produced by Bio-Rad Laboratories, GE Healthcare, Alpha Innotech, FujiFilm Life Science, Licor Biosciences, UVP and Syngene.

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Applications
Peptide Microarray
PubMed
(). Elevated SLC7A2 expression is associated with an abnormal neuroinflammatory response and nitrosative stress in Huntington’s disease. J Neuroinflammation.
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WB, IB, PCA
PubMed
(). Excitotoxic glutamate levels cause the secretion of resident endoplasmic reticulum proteins. J Neurochem.
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IP, Co-IP; WB, IB, PCA
PubMed
(). Full-length MSP1 is a major target of protective immunity after controlled human malaria infection. Life Sci Alliance.
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Peptide Microarray
PubMed
(). The RNA binding protein IGF2BP2/IMP2 alters the cargo of cancer cell-derived extracellular vesicles supporting tumor-associated macrophages. Cell Commun Signal.
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WB, IB, PCA
PubMed
(). The OTX2 Gene Induces Tumor Growth and Triggers Leptomeningeal Metastasis by Regulating the mTORC2 Signaling Pathway in Group 3 Medulloblastomas. Int J Mol Sci.
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WB, IB, PCA
PubMed
(). Structural basis for RNA polymerase II ubiquitylation and inactivation in transcription-coupled repair. Nat Struct Mol Biol.
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WB, IB, PCA
PubMed
(). Reduced progranulin increases tau and α-synuclein inclusions and alters mouse tauopathy phenotypes via glucocerebrosidase. Nat Commun.
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WB, IB, PCA
PubMed
(). Widespread monoclonal IgE antibody convergence to an immunodominant, proanaphylactic Ara h 2 epitope in peanut allergy. J Allergy Clin Immunol.
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Microarray, Microarray chip
PubMed
(). Antigen-Heterologous Vaccination Regimen Triggers Alternate Antibody Targeting in SARS-CoV-2-DNA-Vaccinated Mice. Vaccines (Basel).
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Microarray, Microarray chip
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Microarray, Microarray chip
PubMed
(). Amino-terminal proteolytic fragment of the axon growth inhibitor Nogo-A (Rtn4A) is upregulated by injury and promotes axon regeneration. J Biol Chem.
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WB, IB, PCA
PubMed
(). A zebrafish model of combined saposin deficiency identifies acid sphingomyelinase as a potential therapeutic target. Dis Model Mech.
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WB, IB, PCA
PubMed
(). Conditioning-induced expression of novel glucose transporters in canine skeletal muscle homogenate. PLoS One.
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WB, IB, PCA
PubMed
(). Differential recognition of influenza A virus H1N1 neuraminidase by DNA vaccine-induced antibodies in pigs and ferrets. Front Immunol.
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Peptide Microarray
PubMed
(). Efficacy of a growth hormone-releasing hormone agonist in a murine model of cardiometabolic heart failure with preserved ejection fraction. Am J Physiol Heart Circ Physiol.
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WB, IB, PCA
PubMed
(). Characterization of huntingtin interactomes and their dynamic responses in living cells by proximity proteomics. J Neurochem.
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WB, IB, PCA
PubMed
(). Humoral Immune Response Profile of COVID-19 Reveals Severity and Variant-Specific Epitopes: Lessons from SARS-CoV-2 Peptide Microarray. Viruses.
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Peptide Microarray
PubMed
(). IgE and IgG4 Epitopes of Dermatophagoides and Blomia Allergens before and after Sublingual Immunotherapy. Int J Mol Sci.
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Peptide Microarray
PubMed
(). Identification of Zika Virus NS1-Derived Peptides with Potential Applications in Serological Tests. Viruses.
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Peptide Microarray
PubMed
(). Machine learning-driven multifunctional peptide engineering for sustained ocular drug delivery. Nat Commun.
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Peptide Microarray
PubMed
(). Peptide microarray analysis of in-silico predicted B-cell epitopes in SARS-CoV-2 sero-positive healthcare workers in Bulawayo, Zimbabwe. Acta Trop.
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Peptide Microarray
PubMed
(). The transcription factor TCFL5 responds to A-MYB to elaborate the male meiotic program in mice. Reproduction.
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WB, IB, PCA
PubMed
(). Neuronal transcriptome, tau and synapse loss in Alzheimer's knock-in mice require prion protein. Alzheimers Res Ther.
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WB, IB, PCA
PubMed
(). A Candidate DNA Vaccine Encoding the Native SARS-CoV-2 Spike Protein Induces Anti-Subdomain 1 Antibodies. Vaccines (Basel).
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Microarray, Microarray chip
PubMed
(). A-MYB/TCFL5 regulatory architecture ensures the production of pachytene piRNAs in placental mammals. RNA.
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WB, IB, PCA
PubMed
(). XPC-PARP complexes engage the chromatin remodeler ALC1 to catalyze global genome DNA damage repair. Nature Communications
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WB, IB, PCA
PubMed
(). Multiplex peptide microarray profiling of antibody reactivity against neglected tropical diseases derived B-cell epitopes for serodiagnosis in Zimbabwe. PloS One
Applications
Blocking
PubMed
(). Development and Optimization of Bifunctional Fusion Proteins to Locally Modulate Complement Activation in Diseased Tissue. Frontiers in Immunology
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Blocking
PubMed
(). Tofacitinib Downregulates TNF and Poly(I:C)-Dependent MHC-II Expression in the Colonic Epithelium. Frontiers in Immunology
Applications
WB, IB, PCA; Blocking
PubMed
(). AURKA and PLK1 inhibition selectively and synergistically block cell cycle progression in diffuse midline glioma. IScience
Applications
WB, IB, PCA
PubMed
(). Pairing-Dependent Plasticity in a Dissected Fly Brain Is Input-Specific and Requires Synaptic CaMKII Enrichment and Nighttime Sleep. The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Applications
WB, IB, PCA
PubMed
(). Development of broadly neutralizing antibodies targeting the cytomegalovirus subdominant antigen gH. Communications Biology
Applications
Microarray, Microarray chip; Blocking
PubMed
(). Inhibition of lung microbiota-derived proapoptotic peptides ameliorates acute exacerbation of pulmonary fibrosis. Nature Communications
Applications
Peptide Microarray
PubMed
(). miR-376a-3p and miR-376b-3p overexpression in Hutchinson-Gilford progeria fibroblasts inhibits cell proliferation and induces premature senescence. IScience
Applications
Blocking
PubMed
(). Macrophage IL-1β promotes arteriogenesis by autocrine STAT3- and NF-κB-mediated transcription of pro-angiogenic VEGF-A. Cell Reports
Applications
Blocking
PubMed
(). Enhanced NCLX-dependent mitochondrial Ca2+ efflux attenuates pathological remodeling in heart failure. J Mol Cell Cardiol.
Applications
WB, IB, PCA
PubMed
(). In vitro evolution of myc- tag antibodies: in-depth specificity and affinity analysis of Myc1-9E10 and Hyper-Myc. Biol Chem.
Applications
Peptide Microarray
PubMed
(). Glutamine uptake and catabolism is required for myofibroblast formation and persistence. J Mol Cell Cardiol.
Applications
WB, IB, PCA
PubMed
(). GTSF1 accelerates target RNA cleavage by PIWI-clade Argonaute proteins. Nature.
Applications
WB, IB, PCA
PubMed
(). The SARS-CoV-2 spike protein binds and modulates estrogen receptors. Sci Adv.
Applications
IF, Confocal Microscopy
PubMed
(). UPF1 mutants with intact ATPase but deficient helicase activities promote efficient nonsense-mediated mRNA decay. Nucleic Acids Res.
Applications
WB, IB, PCA
PubMed
(). Rabphilin3A reduces integrin-dependent growth cone signaling to restrict axon regeneration after trauma. Exp Neurol.
Applications
WB, IB, PCA
PubMed
(). Novel colchicine derivative CR42-24 demonstrates potent anti-tumor activity in urothelial carcinoma. Cancer Lett.
Applications
WB, IB, PCA
PubMed
(). HI-NESS: a family of genetically encoded DNA labels based on a bacterial nucleoid-associated protein. Nucleic Acids Research
Applications
WB, IB, PCA
PubMed
(). Automated Laser-Transfer Synthesis of High-Density Microarrays for Infectious Disease Screening. Adv Mater.
Applications
Blocking
PubMed
(). Anti-neuronal antibodies against brainstem antigens are associated with COVID-19. EBioMedicine.
Applications
Peptide Microarray
PubMed
(). Antibody Response to HML-2 May Be Protective in Amyotrophic Lateral Sclerosis. Ann Neurol.
Applications
Peptide Microarray
PubMed
(). A new mechanism of fibronectin fibril assembly revealed by live imaging and super-resolution microscopy. J Cell Sci.
Applications
Peptide Microarray
PubMed
(). Analysis of the Immune Response and Identification of Antibody Epitopes Against the Sigma C Protein of Avian Orthoreovirus Following Immunization with Live or Inactivated Vaccines. Avian Dis.
Applications
Microarray, Microarray chip
PubMed
(). Overcoming culture restriction for SARS-CoV-2 in human cells facilitates the screening of compounds inhibiting viral replication. Antimicrob Agents Chemother.
Applications
WB, IB, PCA
PubMed
(). High level production of amorphadiene using Bacillus subtilis as an optimized terpenoid cell factory. N Biotechnol.
Applications
WB, IB, PCA
PubMed
(). Nutrient transceptors physically interact with the yeast S6/Protein kinase B homolog, Sch9, a TOR kinase target. Biochem J.
Applications
WB, IB, PCA
PubMed
(). Effect of conditioning and physiology hyperthermia on canine skeletal muscle mitochondrial oxygen consumption. J Appl Physiol (1985).
Applications
WB, IB, PCA
PubMed
(). Comparison of Monoclonal Gammopathies Linked to Poliovirus or Coxsackievirus vs. Other Infectious Pathogens. Cells.
Applications
Microarray
PubMed
(). Prenatal Iron Deficiency and Choline Supplementation Interact to Epigenetically Regulate Jarid1b and Bdnf in the Rat Hippocampus into Adulthood. Nutrients
Applications
WB, IB, PCA
PubMed
(). Fasting and fasting-mimicking treatment activate SIRT1/LXRα and alleviate diabetes-induced systemic and microvascular dysfunction. Diabetologia.
Applications
IP, Co-IP; WB, IB, PCA
PubMed
(). Astrocyte-derived thrombospondin induces cortical synaptogenesis in a sex-specific manner. eNeuro.
Applications
WB, IB, PCA
PubMed
(). Siglec-8 Signals Through a Non-Canonical Pathway to Cause Human Eosinophil Death In Vitro. Front Immunol.
Applications
WB, IB, PCA
PubMed
(). Probing multivalent carbohydrate-protein interactions with on-chip synthesized glycopeptides using different functionalized surfaces. Front Chem.
Applications
Other
PubMed
(). Growth hormone-releasing hormone agonists ameliorate chronic kidney disease-induced heart failure with preserved ejection fraction. Proc Natl Acad Sci USA.
Applications
WB, IB, PCA
PubMed
(). Identification of a Zika NS2B epitope as a biomarker for severe clinical phenotypes. RSC Med Chem.
Applications
Other
PubMed
(). Small-molecule inhibition of Lats kinases may promote Yap-dependent proliferation in postmitotic mammalian tissues. Nat Commun.
Applications
WB, IB, PCA
PubMed
(). A target-agnostic screen identifies approved drugs to stabilize the endoplasmic reticulum-resident proteome. Cell Rep.
Applications
WB, IB, PCA
PubMed
(). Chemical systems biology reveals mechanisms of glucocorticoid receptor signaling. Nat Chem Biol.
Applications
In-Cell Western (ICW) Assay
PubMed
(). ELOF1 is a transcription-coupled DNA repair factor that directs RNA polymerase II ubiquitylation. Nat Chem Biol.
Applications
WB, IB, PCA
PubMed
(). Epigenetics Identifier screens reveal regulators of chromatin acylation and limited specificity of acylation antibodies. Sci Rep.
Applications
Dot Blot Assay
PubMed
(). Statins and Bempedoic Acid: Different Actions of Cholesterol Inhibitors on Macrophage Activation. Int J Mol Sci.
Applications
WB, IB, PCA
PubMed
(). Terminal modification, sequence, length, and PIWI-protein identity determine piRNA stability. Mol Cell.
Applications
WB, IB, PCA
PubMed
(). ERCC1 mutations impede DNA damage repair and cause liver and kidney dysfunction in patients. J Exp Med.
Applications
WB, IB, PCA
PubMed
(). Fronto-temporal dementia risk gene TMEM106B has opposing effects in different lysosomal storage disorders. Brain Commun.
Applications
WB, IB, PCA
PubMed
(). Peptides of neuron specific enolase as potential ASD biomarkers: From discovery to epitope mapping. Brain Behav Immun.
Applications
Microarray
PubMed
(). Evolutionarily conserved pachytene piRNA loci are highly divergent among modern humans. Nat Ecol Evol.
Applications
WB, IB, PCA
PubMed
(). The Myc tag monoclonal antibody 9E10 displays highly variable epitope recognition dependent on neighboring sequence context. Sci Signal.
Applications
Microarray
PubMed
(). Efficient elimination of primary B-ALL cells in vitro and in vivo using a novel 4-1BB-based CAR targeting a membrane-distal CD22 epitope. J Immunother Cancer.
Applications
Microarray
PubMed
(). Gene-environment interaction promotes Alzheimer's risk as revealed by synergy of repeated mild traumatic brain injury and mouse App knock-in. Neurobiol Dis.
Applications
WB, IB, PCA
PubMed
(). Anti-tumor immunity influences cancer cell reliance upon ATG7. Oncoimmunology.
Applications
WB, IB, PCA
PubMed
(). Neuroadaptations in the dorsal hippocampus underlie cocaine seeking during prolonged abstinence. Proc Natl Acad Sci USA
Applications
WB, IB, PCA
PubMed
(). Epitopes of Naturally Acquired and Vaccine-Induced Anti-Ebola Virus Glycoprotein Antibodies in Single Amino Acid Resolution. Biotechnol J.
Applications
Microarray
PubMed
(). Versicolorin A, a precursor in aflatoxins biosynthesis, is a food contaminant toxic for human intestinal cells. Environ Int.
Applications
WB, IB, PCA
PubMed
(). Expression and Function of Mas-Related G Protein-Coupled Receptor D and Its Ligand Alamandine in Retina. Mol Neurobiol.
Applications
WB, IB, PCA
PubMed
(). A proteolytic C-terminal fragment of Nogo-A (reticulon-4A) is released in exosomes and potently inhibits axon regeneration. J Biol Chem.
Applications
WB, IB, PCA
PubMed
(). The stress-responsive gene GDPGP1/mcp-1 regulates neuronal glycogen metabolism and survival. J Cell Biol.
Applications
WB, IB, PCA
PubMed
(). Pre-clinical characterisation of E2814, a high-affinity antibody targeting the microtubule-binding repeat domain of tau for passive immunotherapy in Alzheimer's disease. Acta Neuropathol Commun.
Applications
Microarray
PubMed
(). Immunization with full-length Plasmodium falciparum merozoite surface protein 1 is safe and elicits functional cytophilic antibodies in a randomized first-in-human trial. NPJ Vaccines.
Applications
Microarray
PubMed
(). Human HMGN1 and HMGN2 are not required for transcription-coupled DNA repair. Sci Rep.
Applications
WB, IB, PCA
PubMed
(). On-Chip Neo-Glycopeptide Synthesis for Multivalent Glycan Presentation. Chemistry.
Applications
In Vitro Binding Assay
PubMed
(). The cooperative action of CSB, CSA, and UVSSA target TFIIH to DNA damage-stalled RNA polymerase II. Nat Commun.
Applications
WB, IB, PCA
PubMed
(). TrkB agonists prevent postischemic emergence of refractory neonatal seizures in mice. JCI Insight.
Applications
WB, IB, PCA
PubMed
(). A cross-reactive mouse monoclonal antibody against rhinovirus mediates phagocytosis in vitro. Sci Rep.
Applications
Microarray
PubMed
(). Hypoxia regulates the degradation of non-nuclear organelles during lens differentiation through activation of HIF1a Exp Eye Res.
Applications
WB, IB, PCA
PubMed
(). Expression of different L1 isoforms of Mastomys natalensis papillomavirus as mechanism to circumvent adaptive immunity. Elife.
Applications
Microarray
PubMed
(). Identification of truncated C-terminal fragments of the Alzheimer's disease amyloid protein precursor derived from sequential proteolytic pathways. J Neurochem.
Applications
WB, IB, PCA
PubMed
(). Small-molecule inhibition of Lats kinases promotes Yap-dependent proliferation in postmitotic mammalian tissues. bioRxiv
Applications
WB, IB, PCA
Source
(). Cardiac Cell-Derived Matrices Impart Age-Specific Functional Properties to Human Cardiomyocytes. bioRxiv
Applications
WB, IB, PCA
Source
(). Trem2 Deletion Reduces Late-Stage Amyloid Plaque Accumulation, Elevates the Aβ42:Aβ40 Ratio, and Exacerbates Axonal Dystrophy and Dendritic Spine Loss in the PS2APP Alzheimer's Mouse Model. J Neurosci.
Applications
Other
PubMed
(). SNX27 regulates DRA activity and mediates its direct recycling by PDZ-interaction in early endosomes at the apical pole of Caco2 cells. Am J Physiol Gastrointest Liver Physiol.
Applications
WB, IB, PCA
PubMed
(). Organic dust induced mitochondrial dysfunction could be targeted via cGAS-STING or mitochondrial NOX-2 inhibition. bioRxiv
Applications
WB, IB, PCA
Source
(). Fyn-tau Ablation Modifies PTZ-Induced Seizures and Post-seizure Hallmarks of Early Epileptogenesis. Frontiers in Cellular Neuroscience
Applications
WB, IB, PCA
PubMed
(). Thermus thermophilus Argonaute Functions in the Completion of DNA Replication. Cell
Applications
WB, IB, PCA
PubMed
(). In vivo proximity proteomics of nascent synapses reveals a novel regulator of cytoskeleton-mediated synaptic maturation. Nature Communications
Applications
IF, Confocal Microscopy; IHC, ICC, Histology
PubMed
(). Amplified Host Defense by Toll-Like Receptor-Mediated Downregulation of the Glucocorticoid-Induced Leucine Zipper (GILZ) in Macrophages. Frontiers in Immunology
Applications
WB, IB, PCA
PubMed
(). Alzheimer's Disease Risk Factor Pyk2 Mediates Amyloid-β-Induced Synaptic Dysfunction and Loss. J Neurosci.
Applications
WB, IB, PCA
PubMed
(). Lipidomic Analysis of α-Synuclein Neurotoxicity Identifies Stearoyl CoA Desaturase as a Target for Parkinson Treatment. Mol Cell.
Applications
WB, IB, PCA
PubMed
(). Maelstrom Represses Canonical Polymerase II Transcription within Bi-directional piRNA Clusters in Drosophila melanogaster. Mol Cell.
Applications
WB, IB, PCA
PubMed
(). Pyk2 Signaling through Graf1 and RhoA GTPase Is Required for Amyloid-β Oligomer-Triggered Synapse Loss. J Neurosci.
Applications
WB, IB, PCA
PubMed
(). Rescue of Transgenic Alzheimer's Pathophysiology by Polymeric Cellular Prion Protein Antagonists. Cell Rep.
Applications
WB, IB, PCA
PubMed
(). Plexina2 and CRMP2 Signaling Complex Is Activated by Nogo-A-Liganded Ngr1 to Restrict Corticospinal Axon Sprouting after Trauma. J Neurosci.
Applications
WB, IB, PCA
PubMed
(). High-throughput epitope profiling of antibodies in the plasma of Alzheimer's disease patients using random peptide microarrays. Sci Rep.
Applications
Microarray
PubMed
(). Anti-PrPC antibody rescues cognition and synapses in transgenic alzheimer mice. Ann Clin Transl Neurol.
Applications
WB, IB, PCA
PubMed
(). Immunization of cats to induce neutralizing antibodies against Fel d 1, the major feline allergen in human subjects. J Allergy Clin Immunol.
Applications
Microarray
PubMed
(). The RNA-Binding ATPase, Armitage, Couples piRNA Amplification in Nuage to Phased piRNA Production on Mitochondria. Mol Cell.
Applications
WB, IB, PCA
PubMed
(). A Monoclonal Antibody to M-Phase Phosphoprotein 1/Kinesin-Like Protein KIF20B. Monoclon Antib Immunodiagn Immunother.
Applications
Microarray
PubMed
(). Toll-Like Receptor 2 Release by Macrophages: An Anti-inflammatory Program Induced by Glucocorticoids and Lipopolysaccharide. Front Immunol.
Applications
WB, IB, PCA
PubMed
(). Mitochondrial calcium exchange links metabolism with the epigenome to control cellular differentiation. Nat Commun.
Applications
WB, IB, PCA
PubMed
(). Biphasic Response of Protein Kinase A to Cyclic Adenosine Monophosphate Triggers Distinct Epithelial Phenotypes. bioRxiv
Applications
WB, IB, PCA
Source
(). MICU1 regulates mitochondrial cristae structure and function independent of the mitochondrial calcium uniporter channel. bioRxiv
Applications
WB, IB, PCA
Source
(). Fyn kinase regulates misfolded α-synuclein uptake and NLRP3 inflammasome activation in microglia. The Journal of Experimental Medicine
Applications
WB, IB, PCA
PubMed
(). Gesicle-mediated delivery of CRISPR/Cas9 ribonucleoprotein complex for inactivating the HIV provirus. Mol Ther.
Applications
WB, IB, PCA
PubMed
(). MitoRibo-Tag Mice Provide a Tool for In Vivo Studies of Mitoribosome Composition. Cell Reports
Applications
WB, IB, PCA
PubMed
(). Plug-and-Play Protein Modification Using Homology-Independent Universal Genome Engineering. Neuron
Applications
WB, IB, PCA
PubMed
(). Rescue of Transgenic Alzheimer's Pathophysiology by Polymeric Cellular Prion Protein Antagonists. Cell Reports
Applications
WB, IB, PCA
PubMed
(). mitoTev-TALE: a monomeric DNA editing enzyme to reduce mutant mitochondrial DNA levels. EMBO Molecular Medicine
Applications
WB, IB, PCA
PubMed
(). Identification of the antigenic epitopes of maternal autoantibodies in autism spectrum disorders. Brain Behav Immun.
Applications
Microarray
PubMed
(). The nociceptin receptor inhibits axonal regeneration and recovery from spinal cord injury. Sci Signal.
Applications
WB, IB, PCA
PubMed
(). Protein Corona in Response to Flow: Effect on Protein Concentration and Structure. Biophys J.
Applications
WB, IB, PCA
PubMed
(). Mycobacterium tuberculosis-specific CD4+ and CD8+ T cells differ in their capacity to recognize infected macrophages. PLoS Pathog.
Applications
WB, IB, PCA
PubMed
(). BNIP3L/NIX is required for elimination of mitochondria, endoplasmic reticulum and Golgi apparatus during eye lens organelle-free zone formation. Exp Eye Res.
Applications
WB, IB, PCA
PubMed
(). Combinatorial Synthesis of Macromolecular Arrays by Microchannel Cantilever Spotting (µCS). Adv Mater.
Applications
Microarray
PubMed
(). Thrombospondin receptor α2δ-1 promotes synaptogenesis and spinogenesis via postsynaptic Rac1. J Cell Biol.
Applications
WB, IB, PCA
PubMed
(). A Single Mechanism of Biogenesis, Initiated and Directed by PIWI Proteins, Explains piRNA Production in Most Animals. Mol Cell.
Applications
WB, IB, PCA
PubMed
(). Generation and characterization of monoclonal antibodies that recognize human and murine supervillin protein isoforms. PLoS One.
Applications
Microarray
PubMed
(). Liquid and Hydrogel Phases of PrPC Linked to Conformation Shifts and Triggered by Alzheimer's Amyloid-β Oligomers. Mol Cell.
Applications
WB, IB, PCA
PubMed
(). KDEL Receptors Are Differentially Regulated to Maintain the ER Proteome under Calcium Deficiency. Cell Rep.
Applications
WB, IB, PCA
PubMed
(). Development of Neutralizing and Non-neutralizing Antibodies Targeting Known and Novel Epitopes of TcdB of Clostridioides difficile. Front Microbiol.
Applications
Microarray
PubMed
(). Antiviral Effect of Ribavirin against HCV Associated with Increased Frequency of G-to-A and C-to-U Transitions in Infectious Cell Culture Model. Scientific Reports
Applications
WB, IB, PCA
PubMed
(). ICE1 promotes the link between splicing and nonsense-mediated mRNA decay. ELife
Applications
WB, IB, PCA
PubMed
(). Interaction of the Human Respiratory Syncytial Virus matrix protein with cellular adaptor protein complex 3 plays a critical role in trafficking. PLOS One
Applications
WB, IB, PCA
PubMed
(). Early Activation of Experience-Independent Dendritic Spine Turnover in a Mouse Model of Alzheimer's Disease. Cereb Cortex.
Applications
WB, IB, PCA
PubMed
(). Analysis of Cohesin Function in Gene Regulation and Chromatin Organization in Interphase. Methods Mol Biol.
Applications
WB, IB, PCA
PubMed
(). Opposing effects of progranulin deficiency on amyloid and tau pathologies via microglial TYROBP network. Acta Neuropathol.
Applications
WB, IB, PCA
PubMed
(). Induction of Immune Tolerance to Foreign Protein via Adeno-Associated Viral Vector Gene Transfer in Mid-Gestation Fetal Sheep. PLoS One.
Applications
E, EIA
PubMed
(). A biomimetic gelatin-based platform elicits a pro-differentiation effect on podocytes through mechanotransduction. Sci Rep.
Applications
WB, IB, PCA
PubMed
(). Lifespan differences between queens and workers are not explained by rates of molecular damage. Exp Gerontol.
Applications
WB, IB, PCA
PubMed
(). The C Terminus of the Herpes Simplex Virus UL25 Protein Is Required for Release of Viral Genomes from Capsids Bound to Nuclear Pores. J Virol.
Applications
WB, IB, PCA
PubMed
(). Nanoparticle-induced oxidation of corona proteins initiates an oxidative stress response in cells. Nanoscale.
Applications
WB, IB, PCA
PubMed
(). Loss of TMEM106B Ameliorates Lysosomal and Frontotemporal Dementia-Related Phenotypes in Progranulin-Deficient Mice. Neuron.
Applications
WB, IB, PCA
PubMed
(). Systematic and Quantitative Assessment of Hydrogen Peroxide Reactivity With Cysteines Across Human Proteomes. Mol Cell Proteomics.
Applications
WB, IB, PCA
PubMed
(). Conditional Deletion of Prnp Rescues Behavioral and Synaptic Deficits after Disease Onset in Transgenic Alzheimer's Disease. J Neurosci.
Applications
WB, IB, PCA
PubMed
(). Identification of two conserved B-cell epitopes in the gp90 of reticuloendothelial virus using peptide microarray. Vet Microbiol.
Applications
Microarray
PubMed
(). Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses. ACS Omega.
Applications
Microarray
PubMed
(). Expression of endogenous mouse APP modulates β-amyloid deposition in hAPP-transgenic mice. Acta Neuropathologica Communications
Applications
WB, IB, PCA
PubMed
(). Metabotropic glutamate receptor 5 couples cellular prion protein to intracellular signalling in Alzheimer's disease. Brain.
Applications
WB, IB, PCA
PubMed
(). Serum peptide reactivities may distinguish neuromyelitis optica subgroups and multiple sclerosis. Neurol Neuroimmunol Neuroinflamm.
Applications
Microarray
PubMed
(). Identification of a Highly Conserved Epitope on Avian Influenza Virus Non-Structural Protein 1 Using a Peptide Microarray. PLoS One.
Applications
Microarray
PubMed
(). Assembly Dynamics and Stoichiometry of the Apoptosis Signal-regulating Kinase (ASK) Signalosome in Response to Electrophile Stress. Mol Cell Proteomics.
Applications
WB, IB, PCA
PubMed
(). High-Density Peptide Arrays for Malaria Vaccine Development. Methods Mol Biol.
Applications
WB, IB, PCA
PubMed
(). Properties of Two Enterovirus Antibodies that are Utilized in Diabetes Research. Sci Rep.
Applications
Microarray
PubMed
(). High-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in solid matrix material. Nat Commun.
Applications
Other
PubMed
(). Oligomers of Amyloid β Prevent Physiological Activation of the Cellular Prion Protein-Metabotropic Glutamate Receptor 5 Complex by Glutamate in Alzheimer Disease. J Biol Chem.
Applications
WB, IB, PCA
PubMed
(). A single amino acid substitution alter antigenicity of Glycosylated protein 4 of HP-PRRSV. Virol J.
Applications
Microarray
PubMed
(). Transient Hepatic Overexpression of Insulin-Like Growth Factor 2 Induces Free Cholesterol and Lipid Droplet Formation. Front Physiol.
Applications
WB, IB, PCA
PubMed
(). Increased Intimal Hyperplasia After Vascular Injury in Male Androgen Receptor-Deficient Mice. Endocrinology.
Applications
WB, IB, PCA
PubMed
(). Identification of novel antigens contributing to autoimmunity in cardiovascular diseases. Clin Immunol.
Applications
Microarray
PubMed
(). Validation of Cross-Species Reactivity of the VEGF-A/PDGFRβ Bifunctional Antibody PF-06653157. J Ocul Pharmacol Ther.
Applications
WB, IB, PCA
PubMed
(). Dynamic Phosphorylation of Apoptosis Signal Regulating Kinase 1 (ASK1) in Response to Oxidative and Electrophilic Stress. Chem Res Toxicol.
Applications
WB, IB, PCA
PubMed
(). TiO2 Nanoparticles Alter the Expression of Peroxiredoxin Antioxidant Genes. J. Phys. Chem. C
Applications
WB, IB, PCA
Source
(). Beneficial Effects of Combined AT1 Receptor/Neprilysin Inhibition (ARNI) Versus AT1 Receptor Blockade Alone in the Diabetic Eye. Investigative Ophthalmology & Visual Science
Applications
WB, IB, PCA
PubMed
(). Tubulin C-terminal Post-translational Modifications Do Not Occur in Wood Forming Tissue of Populus. Frontiers in Plant Science
Applications
WB, IB, PCA
PubMed
(). Transient Hepatic Overexpression of Insulin-Like Growth Factor 2 Induces Free Cholesterol and Lipid Droplet Formation. Frontiers in Physiology
Applications
WB, IB, PCA
PubMed
(). Printed peptide arrays identify prognostic TNC serumantibodies in glioblastoma patients. Oncotarget
Applications
Microarray
PubMed
(). Proteomic analysis of physiological versus pathological cardiac remodeling in animal models expressing mutations in myosin essential light chains. Journal of Muscle Research and Cell Motility
Applications
WB, IB, PCA
PubMed
(). Pattern recognition receptors in the gut: analysis of their expression along the intestinal tract and the crypt/villus axis. Physiol Rep.
Applications
WB, IB, PCA
PubMed
(). Spot peptide arrays and SPR measurements: throughput and quantification in antibody selectivity studies. J Mol Recognit.
Applications
Microarray
PubMed
(). Cross-Talk between Carbon Metabolism and the DNA Damage Response in S. cerevisiae. Cell Rep.
Applications
WB, IB, PCA
PubMed
(). Biophysical characterization data on Aβ soluble oligomers produced through a method enabling prolonged oligomer stability and biological buffer conditions. Data in Brief
Applications
WB, IB, PCA
PubMed
(). Antiviral Activity of Gold/Copper Sulfide Core/Shell Nanoparticles against Human Norovirus Virus-Like Particles. PloS One
Applications
WB, IB, PCA
PubMed
(). Huntingtin is required for normal excitatory synapse development in cortical and striatal circuits. J Neurosci.
Applications
WB, IB, PCA
PubMed
(). Lysosome size, motility and stress response regulated by fronto-temporal dementia modifier TMEM106B. Mol Cell Neurosci.
Applications
WB, IB, PCA
PubMed
(). Isolation and characterization of the herpes simplex virus 1 terminase complex. J Virol.
Applications
WB, IB, PCA
PubMed
(). Analysis of autoantibodies to 3-hydroxy-3-methylglutaryl-coenzyme A reductase using different technologies. J Immunol Res.
Applications
Microarray
PubMed
(). Antigenic characteristics of glycosylated protein 3 of highly pathogenic porcine reproductive and respiratory syndrome virus. Virus Res.
Applications
Microarray
PubMed
(). Redox processes inform multivariate transdifferentiation trajectories associated with TGFβ-induced epithelial-mesenchymal transition. Free Radic Biol Med.
Applications
Other
PubMed
(). Therapeutic molecules and endogenous ligands regulate the interaction between brain cellular prion protein (PrPC) and metabotropic glutamate receptor 5 (mGluR5). J Biol Chem.
Applications
WB, IB, PCA
PubMed
(). Linear epitope recognition antibodies strongly respond to the C-terminal domain of HP-PRRSV GP5. Vet Microbiol.
Applications
Microarray
PubMed
(). Autophagy-dependent PELI3 degradation inhibits proinflammatory IL1B expression. Autophagy.
Applications
WB, IB, PCA
PubMed
(). Astrocytes refine cortical connectivity at dendritic spines. Elife.
Applications
WB, IB, PCA
PubMed
(). SUMOylation regulates the SNF1 protein kinase. Proc Natl Acad Sci U S A.
Applications
WB, IB, PCA
PubMed
(). WhiB7, an Fe-S-dependent transcription factor that activates species-specific repertoires of drug resistance determinants in actinobacteria. J Biol Chem.
Applications
WB, IB, PCA
PubMed
(). Mutational analysis of sclerostin shows importance of the flexible loop and the cystine-knot for Wnt-signaling inhibition. PLoS One.
Applications
Cellular assay
PubMed
(). Alzheimer amyloid-β oligomer bound to postsynaptic prion protein activates Fyn to impair neurons. Nat Neurosci.
Applications
WB, IB, PCA
PubMed
(). Sensing immune responses with customized peptide microarrays. Biointerphases.
Applications
Microarray
PubMed
(). Rapamycin attenuates the expression of cocaine-induced place preference and behavioral sensitization. Addiction Biology
Applications
WB, IB, PCA
PubMed
(). Residues of the UL25 protein of herpes simplex virus that are required for its stable interaction with capsids. J Virol.
Applications
WB, IB, PCA
PubMed
(). Endo-lysosomal vesicles positive for Rab7 and LAMP1 are terminal vesicles for the transport of dextran. PLoS One.
Applications
WB, IB, PCA
PubMed
(). Coordinated regulation of extracellular matrix synthesis by the microRNA-29 family in the trabecular meshwork. Invest Ophthalmol Vis Sci.
Applications
WB, IB, PCA
PubMed
(). Firefly luciferase and RLuc8 exhibit differential sensitivity to oxidative stress in apoptotic cells. PloS One
Applications
WB, IB, PCA
PubMed
(). High-content screening for compounds that affect mtDNA-encoded protein levels in eukaryotic cells. J Biomol Screen.
Applications
WB, IB, PCA
PubMed
(). Evaluation of the cytotoxic effects of ophthalmic solutions containing benzalkonium chloride on corneal epithelium using an organotypic 3-D model. BMC Ophthalmol.
Applications
WB, IB, PCA
PubMed
(). Astaxanthin reduces ischemic brain injury in adult rats. FASEB J,
Applications
WB, IB, PCA
PubMed

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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