Sandwich ELISA using RM123 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (κ+ λ) antibody RM129 as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin. ELISA of human immunoglobulins shows RM123 reacts to IgD from human plasma and IgDκ from human myeloma. No cross reactivity with human IgG, IgM, IgA, or IgE. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM123 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. A titer ELISA using RM123. The plate was coated with different amounts of human IgD (from plasma). A serial dilution of RM123 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
Product Details Product Name Biotin Anti-Human IgD Rabbit Monoclonal Antibody, Clone RM123 Product Description Biotinylated Rabbit monoclonal to Human IgD Catalog No. 31-1025-02 Clone Name RM123 Product Data Sheet Biotin Anti-Human IgD Rabbit Monoclonal Antibody Data Sheet RM123 Specificity RM123 reacts to human IgD. No cross reactivity with human IgG, IgM, IgE, or IgA. Immunogen Human IgD Application ELISA Species Reactivity Human Conjugate Biotin Intended Use Research Use Only . Properties Form Liquid Storage Instructions Store at -20.0°C Stability Stable for 1 Year at -20.0°C from date of receipt Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide Volume/Item Size 50 µg Concentration 1.0 mg/mL Purity Protein A affinity purified from an animal origin–free culture supernatant Clone Type Monoclonal Isotype Rabbit IgG Usage ELISA: 25 ng/well – 200 ng/well (for Capture);
0.01 µg/mL – 0.1 µg/mL (for Detection); Keywords . Product Images
Biotin Anti-Human IgD rabbit monoclonal antibody [RM123]
![Biotin Anti-Human IgD rabbit monoclonal antibody [RM123]](https://www.biovalley.fr/upload/-img-lapin1.png)
Référence 31-1025-02
Conditionnement : 50ug
Marque : RevMab Biosciences
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Téléphone : +1 850 650 7790
Biotinylated Rabbit monoclonal to Human IgD
50 µg
