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HeLa cell cultures were stained with MO22159 antibody (green). The antibody stains spindle poles and mitotic spindles at anaphase and concentrates on the midbody between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Cells were counterstained with our chicken polyclonal antibody to Vimentin CH22108 red. Blue is a DNA stain.
HeLa cell cultures were stained with MO22159 antibody (green). The antibody stains spindle poles and mitotic spindles at anaphase and concentrates on the midbody between the two daughter cells during telophase. It is therefore a useful marker of dividing cells. Cells were counterstained with our chicken polyclonal antibody to Vimentin CH22108 red. Blue is a DNA stain.
L: Western blot analysis of MO22159 in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with MO22159, which binds strongly to a band at about 46 kDa, which is Aurora A and also shows binding to a band at 38 kDa. R: Blots of recombinant human Aurora A, B and C were probed with MO22159, which binds to both Aurora A and B. We conclude that the 38 kDa band seen in the HeLa extract is Aurora B.
L: Western blot analysis of MO22159 in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with MO22159, which binds strongly to a band at about 46 kDa, which is Aurora A and also shows binding to a band at 38 kDa. R: Blots of recombinant human Aurora A, B and C were probed with MO22159, which binds to both Aurora A and B. We conclude that the 38 kDa band seen in the HeLa extract is Aurora B.