Western Blot (Cell lysate)
Western blot analysis of ATG5 in mouse wildtype ES cell lysate (Lane 1) using ATG5 polyclonal antibody (Cat # PAB12482). Lane 2 : ATG5 KO ES cell lysate (negative control). Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.
Immunohistochemistry
Staining of ATG5 in human liver hepatocytes using ATG5 polyclonal antibody (Cat # PAB12482) at 2.5 ug/mL .
Immunofluorescence
Immunofluorescent staining of ATG5 on SH-SY5Y cells using ATG5 polyclonal antibody (Cat # PAB12482) at 1 : 250. Photo courtesy of an anonymous collaborator.
- Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ATG5.
Immunogen
A synthetic peptide corresponding to amino acids 1-50 of human ATG5.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
This antibody is selective for the full-length and calpain cleaved isoform proteins. The short isoform is missing a.a. 1-79. The calpain cleaved form of ATG5 is missing a.a. 195-275.
Form
Liquid
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2.5 ug/mL)
Western Blot (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (30% glycerol, 0.09% sodium azide)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot (Cell lysate)
Western blot analysis of ATG5 in mouse wildtype ES cell lysate (Lane 1) using ATG5 polyclonal antibody (Cat # PAB12482). Lane 2 : ATG5 KO ES cell lysate (negative control). Photo courtesy of Dr. Beth Levine, UT Southwestern Medical Center.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry
Staining of ATG5 in human liver hepatocytes using ATG5 polyclonal antibody (Cat # PAB12482) at 2.5 ug/mL .Immunofluorescence
Immunofluorescent staining of ATG5 on SH-SY5Y cells using ATG5 polyclonal antibody (Cat # PAB12482) at 1 : 250. Photo courtesy of an anonymous collaborator. - Gene Info — ATG5
Entrez GeneID
9474Protein Accession#
Q9H1Y0Gene Name
ATG5
Gene Alias
APG5, APG5-LIKE, APG5L, ASP, hAPG5
Gene Description
ATG5 autophagy related 5 homolog (S. cerevisiae)
Omim ID
604261Gene Ontology
HyperlinkGene Summary
S. cerevisiae)-like|APG5 autophagy 5-like|ATG5 autophagy related 5-like|OTTHUMP00000017823|OTTHUMP00000017824|OTTHUMP00000040507|apoptosis specific protein
Other Designations
APG5 (autophagy 5, S. cerevisiae)-like|APG5 autophagy 5-like|ATG5 autophagy related 5-like|OTTHUMP00000017823|OTTHUMP00000017824|OTTHUMP00000040507|apoptosis specific protein
- Interactomes
- Pathways
- Diseases
- Publication Reference
- A key role for autophagy and the autophagy gene Atg16l1 in mouse and human intestinal Paneth cells.
Cadwell K, Liu JY, Brown SL, Miyoshi H, Loh J, Lennerz JK, Kishi C, Kc W, Carrero JA, Hunt S, Stone CD, Brunt EM, Xavier RJ, Sleckman BP, Li E, Mizushima N, Stappenbeck TS, Virgin HW 4th.
Nature 2008 Nov; 456(7219):259.
Application：WB-Ce, Mouse, Mouse intestinal epithelium, MEF cells.
- Compensatory activation of ERK1/2 in Atg5-deficient mouse embryo fibroblasts suppresses oxidative stress-induced cell death.
Pyo JO, Nah J, Kim HJ, Lee HJ, Heo J, Lee H, Jung YK.
Autophagy 2008 Apr; 4(3):315.
Application：WB-Ce, WB-Tr, Human, MEF, SH-SY5Y cells.
- Loss of macroautophagy promotes or prevents fibroblast apoptosis depending on the death stimulus.
Wang Y, Singh R, Massey AC, Kane SS, Kaushik S, Grant T, Xiang Y, Cuervo AM, Czaja MJ.
The Journal of Biological Chemistry 2008 Feb; 283(8):4766.
Application：WB-Ce, Mouse, MEFs.
- A key role for autophagy and the autophagy gene Atg16l1 in mouse and human intestinal Paneth cells.