Anti-Phospho-CDK5-Tyr15 antibody (1-50 aa)

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:5000 IF 1:50-200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	CDK5
Gene ID:	1020
Uniprot ID:	CDK5_HUMAN
Immunogen Region:	1-50 aa
Specificity:	Phospho-Cdk5 (Y15) Polyclonal Antibody detects endogenous levels of Cdk5 protein only when phosphorylated at Y15.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human CDK5 around the phosphorylation site of Tyr15 at the amino acid range 1-50

Post Translational Modifications	Phosphorylation on Tyr-15 by ABL1 and FYN, and on Ser-159 by casein kinase 1 promotes kinase activity. By contrast, phosphorylation at Thr-14 inhibits activity. Phosphorylation at Ser-159 is essential for maximal catalytic activity.
Function	Proline-directed serine/threonine-protein kinase essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. Interacts with D1 and D3-type G1 cyclins. Phosphorylates SRC, NOS3, VIM/vimentin, p35/CDK5R1, MEF2A, SIPA1L1, SH3GLB1, PXN, PAK1, MCAM/MUC18, SEPT5, SYN1, DNM1, AMPH, SYNJ1, CDK16, RAC1, RHOA, CDC42, TONEBP/NFAT5, MAPT/TAU, MAP1B, histone H1, p53/TP53, HDAC1, APEX1, PTK2/FAK1, huntingtin/HTT, ATM, MAP2, NEFH and NEFM. Regulates several neuronal development and physiological processes including neuronal survival, migration and differentiation, axonal and neurite growth, synaptogenesis, oligodendrocyte differentiation, synaptic plasticity and neurotransmission, by phosphorylating key proteins. Negatively regulates the CACNA1B/CAV2.2 -mediated Ca(2+) release probability at hippocampal neuronal soma and synaptic terminals. Activated by interaction with CDK5R1 (p35) and CDK5R2 (p39), especially in postmitotic neurons, and promotes CDK5R1 (p35) expression in an autostimulation loop. Phosphorylates many downstream substrates such as Rho and Ras family small GTPases (e.g. PAK1, RAC1, RHOA, CDC42) or microtubule-binding proteins (e.g. MAPT/TAU, MAP2, MAP1B), and modulates actin dynamics to regulate neurite growth and/or spine morphogenesis. Phosphorylates also exocytosis associated proteins such as MCAM/MUC18, SEPT5, SYN1, and CDK16/PCTAIRE1 as well as endocytosis associated proteins such as DNM1, AMPH and SYNJ1 at synaptic terminals. In the mature central nervous system (CNS), regulates neurotransmitter movements by phosphorylating substrates associated with neurotransmitter release and synapse plasticity.synaptic vesicle exocytosis, vesicles fusion with the presynaptic membrane, and endocytosis. Promotes cell survival by activating anti-apoptotic proteins BCL2 and STAT3, and negatively regulating of JNK3/MAPK10 activity. Phosphorylation of p53/TP53 in response to genotoxic and oxidative stresses enhances its stabilization by preventing ubiquitin ligase-mediated proteasomal degradation, and induces transactivation of p53/TP53 target genes, thus regulating apoptosis. Phosphorylation of p35/CDK5R1 enhances its stabilization by preventing calpain-mediated proteolysis producing p25/CDK5R1 and avoiding ubiquitin ligase-mediated proteasomal degradation. During aberrant cell-cycle activity and DNA damage, p25/CDK5 activity elicits cell-cycle activity and double-strand DNA breaks that precedes neuronal death by deregulating HDAC1. DNA damage triggered phosphorylation of huntingtin/HTT in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity. Phosphorylation of PXN reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Negative regulator of Wnt/beta-catenin signaling pathway. Activator of the GAIT (IFN-gamma-activated inhibitor of translation) pathway, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells.phosphorylates the linker domain of glutamyl-prolyl tRNA synthetase (EPRS) in a IFN-gamma-dependent manner, the initial event in assembly of the GAIT complex. Phosphorylation of SH3GLB1 is required for autophagy induction in starved neurons. Phosphorylation of TONEBP/NFAT5 in response to osmotic stress mediates its rapid nuclear localization. MEF2 is inactivated by phosphorylation in nucleus in response to neurotoxin, thus leading to neuronal apoptosis. APEX1 AP-endodeoxyribonuclease is repressed by phosphorylation, resulting in accumulation of DNA damage and contributing to neuronal death. NOS3 phosphorylation down regulates NOS3-derived nitrite (NO) levels. SRC phosphorylation mediates its ubiquitin-dependent degradation and thus leads to cytoskeletal reorganization. May regulate endothelial cell migration and angiogenesis via the modulation of lamellipodia formation. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-BMAL1 heterodimer in association with altered stability and subcellular distribution.
Protein Name	Cyclin-Dependent Kinase 5 Cell Division Protein Kinase 5 Cyclin-Dependent-Like Kinase 5 Serine/Threonine-Protein Kinase Pssalre Tau Protein Kinase Ii Catalytic Subunit Tpkii Catalytic Subunit
Database Links	Reactome: R-HSA-180024 Reactome: R-HSA-399956 Reactome: R-HSA-6804756 Reactome: R-HSA-8862803 Reactome: R-HSA-9031628 Reactome: R-HSA-9032845 Reactome: R-HSA-9768919 Reactome: R-HSA-983231
Cellular Localisation	Isoform 1: Cytoplasm Nucleus Cell Membrane Peripheral Membrane Protein Perikaryon Cell Projection Lamellipodium Growth Cone Postsynaptic Density Synapse In Axonal Growth Cone With Extension To The Peripheral Lamellipodia Under Neurotoxic Stress And Neuronal Injury Conditions Cdk5r (P35) Is Cleaved By Calpain To Generate Cdk5r1 (P25) In Response To Increased Intracellular Calcium The Elevated Level Of P25 When In Complex With Cdk5 Leads To Its Subcellular Misallocation As Well As Its Hyperactivation Colocalizes With Ctnnd2 In The Cell Body Of Neuronal Cells And With Ctnnb1 In The Cell-Cell Contacts And Plasma Membrane Of Undifferentiated And Differentiated Neuroblastoma Cells Reversibly Attached To The Plasma Membrane In An Inactive Form When Complexed To Dephosphorylated P35 Or Cdk5r2 (P39) P35 Phosphorylation Releases This Attachment And Activates Cdk5 Isoform 2: Nucleus
Alternative Antibody Names	Anti-Cyclin-Dependent Kinase 5 antibody Anti-Cell Division Protein Kinase 5 antibody Anti-Cyclin-Dependent-Like Kinase 5 antibody Anti-Serine/Threonine-Protein Kinase Pssalre antibody Anti-Tau Protein Kinase Ii Catalytic Subunit antibody Anti-Tpkii Catalytic Subunit antibody Anti-CDK5 antibody Anti-CDKN5 antibody Anti-PSSALRE antibody

Information sourced from Uniprot.org

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