Polysaccharide Arabinogalactan-protein (AGP) from Oryza sativa
Host:
Rat
Clonality:
Monoclonal
Purity:
Cell Culture Supernatant, with 0.05% sodium azide.
Format:
Liquid
Quantity:
1 ml
Storage:
Store at -20°C. Make aliquots to avoid repeated freeze-thaw cycles, Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Tested applications:
Immunofluorescence (IF)
Recommended dilution:
1: 10 (IF)
Reactivity
Confirmed reactivity:
Higher plants
Additional Information
Additional information (application):
Antibody is recognizing carbohydrate epitope containing Β-linked glucuronic acid.
Background
Background:
The plant cell wall surrounds the plant cell as a complex network of polysaccharides classed as: cellulose, hemicelluloses and pectic polysaccharides and glycoproteins. Anchored to or embedded into plant cell wall are other polymers, like: lignin, suberin or cutin. Arabinogalactans can be found in plants as free glycans, or attached to rhamnogalacturonan-I or protein backbones.
Protocols
Immunofluorsecence as described in: Developmental complexity of arabinan polysaccharides and their processing in plant cell walls Verhertbruggen Y, Marcus SE, Haeger A, Verhoef R, Schols HA, McCleary BV, McKee L, Gilbert HJ, Knox JP., The Plant Journal (2009) 59, 413–425.
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