Alkaline Phosphatase (ALP) Activity Assay Kit (PNPP method)
Référence E-BC-K009-M-500
Conditionnement : 500Assays
Marque : Elabscience
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
| Detection Principle | Under alkaline conditions, alkaline phosphatase catalyzes the hydrolysis of p-nitrobenzene phosphate disodium to produce p-nitrophenol and phosphoric acid. Under strong alkaline conditions, p-nitrophenol is bright yellow and has a maximum absorption peak at 405 nm. Therefore, the activity of ALP can be calculated by measuring the OD value at 405 nm. |
| Synonyms | ALP |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (400-415 nm, optimum wavelength: 405 nm) |
| Research Area | Liver And Renal Function |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.27 U/L |
| Detection Range | 0.27-50.8 U/L |
| Precision | inter-assay CV: 4.6% | intra-assay CV: 1.2% |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 10-20 |
| Mouse serum | 20-30 |
| Rat serum | 10-20 |
| Mouse plasma | 10-20 |
| HepG2 supernatant | 8-12 |
| 10% Rat kidney tissue homogenate | 400-1200 |
| 10% Mouse liver tissue homogenate | 10-15 |
| 10% Rat lung tissue homogenate | 50-100 |
| 10% Mouse brain tissue homogenate | 30-50 |
| Human urine | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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