The Alamar Blue Cell Viability Assay Kit is a kit that detects cell proliferation by the redox indicator Resazurin. Resazurin is oxidized, which is blue in color and basically non-fluorescent. Once entering the cells, resazurin is reduced to resorufin. Resorufin is pink in color and highly red fluorescent. The number of living cells is proportional to the fluorescent or colorimetric signal of resorufin. Therefore, cell viability can be detected by fluorescence- or colorimetric-based instrumentation. But colorimetric detection is not as sensitive as fluorescence detection.

This kit has a wide range of applicability for the proliferation assay of animal cells, plants, bacteria, and fungi. Compared with MTT, WST-1, CCK-8 and other methods, this kit has higher sensitivity, and can detect as low as 50-100 cells per well when using fluorescence. Moreover, this kit is a ready-to-use reagent that is suitable for high-throughput screening. In addition, this reagent is non-toxic and does not affect cell health. For 96-well plates, 5 mL of this reagent is supplied for 500 assays; 25 mL is supplied for 2500 assays; and 100 mL is supplied for 10000 assays.

Figure 1: The Alamar Blue Cell Viability Assay to detect cell proliferation

Figure 2: The Alamar Blue Cell Viability Assay Kit protocol

Figure 3: This product is used with Competitor products (Competitor T) to detect the proliferation of Jurkat cells