Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> Adipogenesis Fluorometric Assay Kit

Adipogenesis Fluorometric Assay Kit

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Référence E-BC-F065-96T

Conditionnement : 96T

Marque : Elabscience

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Téléphone : +1 850 650 7790

Detection Principle Triglycerides were converted by enzyme to produce hydrolytic products, which were catalyzed by the enzyme to produce fluorescent substances. The triglyceride accumulation can be calculated by meas-uring the fluorescence intensity at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.
Sample Type Tissue,Cell
Detection Method Fluorometric method
Detection Instrument Fluorescence microplate reader (Ex/Em=535 nm/587 nm)
Research Area Glycolysis And Lipid Metabolism ,  Energy Metabolism
Storage This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Valid Period 12 months
Sensitivity 1.4 μmol/L
Detection Range 1.4-32 μmol/L
Precision inter-assay CV: 8.4% | intra-assay CV: 0.04
Assay Time 20 min
Calculation Tool Calculation Tool
Cat.No. Product Name Sizes
E-BC-F039 Free Fatty Acids (NEFA/FFA) Fluorometric Assay Kit 96T , 48T
E-BC-F041 Glucose Uptake Fluorometric Assay Kit 500Assays , 96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
10% Porcine heart tissue homogenate 3-6
10% Porcine liver tissue homogenate 8-10
CHO cell (10^6) 1
293T cell (10^6) 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

Other Clones

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Other Formats

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Biotin

Chromogenic Biotin

Cyanine 3

Cyanine 5

Cyanine 5.5

Cyanine 7

Elab Fluor®405

Elab Fluor®430

Elab Fluor®488

Elab Fluor®568

Elab Fluor®594

Elab Fluor®647

Elab Fluor®680

Elab Fluor®700

Elab Fluor®750

Elab Fluor®Red 780

Elab Fluor®Violet 450

FITC

HRP

NHS-Biotin

NHS-LC-LC-Biotin

Sulfo-NHS-Biotin

Sulfo-NHS-LC-LC-Biotin

Unconjugated

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detection instrument

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