Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.
 
> Aconitase(ACO) Activity Assay Kit

Aconitase(ACO) Activity Assay Kit

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Référence E-BC-K656-M-48T

Conditionnement : 48T

Marque : Elabscience

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Detection Principle Aconitase (ACO) is an important Fe/S protein enzyme in cells, which mainly exists in cytoplasm and mitochondria. ACO catalyzes the reversible reaction of citric acid to isocitric acid from the intermediate product cisaconite acid in the cell, which plays an important role through maintaining the tricarboxylic acid cycle and glyoxylic acid cycle. Aconitase catalyzes isocitrate to produce cis-aconitase. Cis-aconitase has a characteristic absorption peak at 240 nm. The activity of this enzyme was calculated by measuring the production rate of cis-aconitase.
Synonyms ACO
Sample Type Animal tissue
Detection Method Colorimetric method
Detection Instrument Microplate reader (230-250 nm, optimum wavelength: 240 nm)
Research Area Tricarboxylic Acid（TCA）Cycle ,  Energy Metabolism
Other Reagents Required Normal saline (0.9% NaCl) , PBS (0.01 mol/L, pH 7.4)
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 2.32 U/L
Detection Range 2.32-60.19 U/L
Precision inter-assay CV: 9.5% | intra-assay CV: 5.9%
Sample Volume 20 μL(tissue homogenate)
Assay Time 25 min
Cat.No. Product Name Sizes
E-BC-K083-M α-Ketoglutarate Dehydrogenase (α-KGDH) Activity Assay Kit 96T
E-BC-K178-M Citrate Synthase (CS) Activity Assay Kit 96T , 48T
E-BC-K650-M Pyruvate Dehydrogenase (PDH) Activity Assay Kit 96T
E-BC-K651-M NAD-Isocitrate Dehydrogenase (NAD-IDH) Activity Assay Kit 96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
10% Rat liver tissue homogenate 6-10
10% Mouse kidney tissue homogenate 4-6
10% Mouse liver tissue homogenate 6-10
10% Mouse brain tissue homogenate 4-6
10% Rat lung tissue homogenate 6-10
10% Mouse muscle tissue homogenate 2-4

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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