β-Glucosidase (β-GC) Activity Assay Kit
Référence E-BC-K822-M-48T
Conditionnement : 48T
Marque : Elabscience
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
| Detection Principle | β-glucosidase (β-GC), also known as β-glucoside hydrolase, can efficiently hydrolyze the terminal non-reducing β-D-glucoside bonds in various glycoside compounds, thereby releasing β-D-glucose and corresponding ligands. The enzyme is widely present in nature, including the seeds of various plants, the small intestine and other organs of animals, and microorganisms. β-GC is a very important industrial enzyme with various applications. In the field of medicine, β-GC catalyzes the degradation of glycoside groups to inhibit the expansion of cancer cells. In terms of diet, β-GC catalyzes the production of anti-cancer molecules isoflavones in soybean. In the energy field, β-GC is one of the key enzymes in the conversion of cellulose to glucose. β-GC catalyzed the reaction of substrate to produce chromogenic substances at the wavelength of 400 nm, the maximum absorption of β-GC enzyme activity was reflected by measuring the OD value at 400 nm. |
| Synonyms | β-GC |
| Sample Type | Tissue,Fungus |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (395-410 nm, optimum wavelength: 400 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Plant Physiology Series |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.06 U/L |
| Detection Range | 0.06-6.79 U/L |
| Precision | inter-assay CV: 2.0-9.7% | intra-assay CV: 2.8-4.5% |
| Sample Volume | 20 μL(tissue homogenate) |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Apple seeds homogenization | 50-60 |
| 10% Spinage tissue homogenization | 1 |
| 10% Shii-take tissue homogenization | 3-5 |
| 10% Grapefruit seeds homogenization | 1 |
| 10% Corn tissue homogenization | 1 |
| 10% Mushroom tissue homogenization | 1 |
| 10% Cabbage tissue homogenization | 1 |
| 10% Mouse small intestine tissue homogenization | 1 |
| 10% Pear seeds homogenization | 2-4 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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